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Lectin binding sites on CD34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study

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Abstract

This study was performed to obtain a better insight into the glycosylation pattern of human CD34+ haematopoietic stem cells and lymphocytes from peripheral blood using an ultrastructural post-embedding technique. Lectins applied were derived from Canavalia ensiformis (Con A), Triticum vulgare (WGA), Lycopersicon esculentum (LEA), Limulus polyphemus (LPA), Ulex europaeus-I (UEA-I), Bauhinia purpurea (BPA), Glycine max (SBA), Helix pomatia (HPA), Arachis hypogaea (PNA) and Erythrina cristagalli (ECA). Our results showed almost identical staining patterns with both CD34+ cells and mature lymphocytes from peripheral blood. Con A displayed a prominent reactivity with the nuclear envelope and a weak staining of the plasma membrane. As demonstrated by an elaborate lectin double-labelling technique, WGA revealed an opposite staining pattern. Following neuraminidase treatment of sections, BPA, PNA and SBA exhibited a prominent staining of the plasma membrane in CD34+ cells and lymphocytes as well. Membrane reactivity with HPA was restricted to the majority of lymphocytes, presumably T-lymphocytes. Infrequently occurring dense cytoplasmic (lysosomal) bodies were reactive with a variety of lectins, and a weak diffuse nuclear labelling was observable with LPA, UEA-I, WGA and Con A. It is tempting to speculate that carbohydrate moieties on plasma membranes may be involved in the complex mechanisms characterizing cell-to-cell interactions (adhesion) and particularly in the so-called phenomenon of homing.

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Authors and Affiliations

  1. Institute of Pathology, University of Cologne, Joseph-Stelzmann-Str. 9, 50924, Cologne, Germany

    Thomas A. Kuemmel, Juergen Thiele, Achim H. Blaeser, Claudia Wickenhauser & Stephan E. Baldus

  2. Institute of Pathology, University of Cologne, Joseph-Stelzm

    Robert Fischer

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  1. Thomas A. Kuemmel
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  2. Juergen Thiele
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  3. Achim H. Blaeser
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  4. Claudia Wickenhauser
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  5. Stephan E. Baldus
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  6. Robert Fischer
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Kuemmel, T.A., Thiele, J., Blaeser, A.H. et al. Lectin binding sites on CD34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study. J Mol Hist 29, 695–705 (1997). https://doi.org/10.1023/A:1026408900534

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