Ecotoxicology

, Volume 8, Issue 3, pp 225–237 | Cite as

Integrated Biological and Chemical Monitoring: In situ Physiological Responses of Freshwater Crayfish to Fluctuations in Environmental Ammonia Concentrations

  • M. J. Bloxham
  • P. J. Worsfold
  • M. H. Depledge
Article

Abstract

A portable, computer-aided physiological monitoring system (CAPMON) has been integrated with an automated, flow injection (FI) based chemical monitor to enable continuous, long-term recording of cardiac activity in selected aquatic organisms, and total ammonia concentration in the surrounding environment. Heart rate of the freshwater crayfish Pacifastacus leniusculus was recorded using non-invasive infrared emitter/detectors to transduce heart beat from 4 animals simultaneously. Data were collected continuously and stored on a laptop computer. The chemical monitor incorporated a gas diffusion unit and a solid state photometric detector. Remote control and data processing were accomplished using an in-house designed microcomputer. The instrumentation was fully evaluated in the laboratory and the field and was shown to be capable of operating unattended for periods of at least 1 week. An exposure-response experiment showed that 4 h exposures to concentrations of ammonia greater than 5 mg l-1 had a significant stimulatory effect on heart rate (ANOVA F=7.6; df=5; P<0.0005). The feasibility of using the system in situ was demonstrated in a 2 week field trial in which the integrated monitors were successfully deployed at a landfill leachate lagoon.

Ammonia in situ monitoring crustacea heart rate flow injection landfill leachate 

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References

  1. Aagaard, A., Andersen, B.B. and Depledge, M.H, (1991). Simultaneous monitoring of physiological and behavioural activity in marine organisms using non-invasive computer-aided techniques. Marine Ecology Progress Series 73, 277–82.Google Scholar
  2. Aagaard, A. (1996). In situ variation in heart rate of the shore crab Carcinus maenas in relation to environmental factors and physiological condition. Marine Biology. 125, 765–72.Google Scholar
  3. Adams, J.R. W., Dolby, J.C. and Williams, P.N. (1992). National Rivers Authority initiatives in the development of portable monitoring equipment. 6, 64–71.Google Scholar
  4. Andrew, K.N., Worsfold, P.J. and Comber, M. (1995). On-line flow injection monitoring of ammonia in industrial liquid effluents. Analytica Chimica Acta 314, 33–43.Google Scholar
  5. Benson, R.L., Worsfold, P.J. and Sweeting, F.W. (1990). On-line determination of residual aluminium in potable and treated waters by flow injection analysis. Analytica Chimica Acta 238, 177–82.Google Scholar
  6. Blundell, N.J., Hopkins, A., Worsfold, P.J. and Casey, H. (1993). A portable, battery powered flow injection monitor for the in situ analysis of nitrate in natural waters. Journal of Automatic Chemistry 15, 159–66.Google Scholar
  7. Blundell, N.J., Worsfold, P.J., Casey, H. and Smith, S. (1995). The design and performance of a portable, automated flow injection monitor for the in situ analysis of nutrients in natural waters. Environment International 21, 205–9.Google Scholar
  8. Borcherding, J. (1992). Another early warning system for the detection of toxic discharges in the aquatic environment based on valve movements of the fresh water mussel Dreissena polymorpha. Limnologie aktuell 4, 127–46.Google Scholar
  9. Clinch, J.R. Worsfold, P.J. and Casey, H. (1987). An automated spectrophotometric field monitor for water quality parameters. Determination of nitrate. Analytica Chimica Acta 200, 523–31.Google Scholar
  10. Depledge, M. H. and Andersen, B.B. (1990). A computer aided physiological monitoring system for continuous, long term recording of cardiac activity in selected invertebrates. Comparative Biochemical Physiology 96A, 473–7.Google Scholar
  11. Depledge, M.H. (1993). The rational basis of the use of biomarkers as ecotoxicological tools. In M. C. Fossi, and C. Leonzio, (eds) Non destructive biomarkers in vertebrates, p. 272. Boca Raton, FL: Lewis.Google Scholar
  12. Evans, G.P. and Solman, A.J. (1985). Continuous monitoring of water quality. Patent application, EP 0.158.522.Google Scholar
  13. Jenner, H.A., Noppert, F. and Sikking, T. (1989). A new system for the detection of valve movement response of bivalves. KEMA Scientific and Technical Report 7, 91–8.Google Scholar
  14. Kramer, K.J.M. and Botterweg, J. (1991). Aquatic biological early warning systems: An overview. In D. W. Jeffery, and B. Madden, (eds) Bioindicators and Environmental Management, p. 95. London: Academic Press.Google Scholar
  15. Kramer, K.J.M., Jenner, H.A. and De Zwart, D. (1989). The valve movement response of mussels: a tool in biological monitoring. Hydrobiologia 188/189, 433–43.Google Scholar
  16. Muraki, H., Higuchi, K., Sasaki, M., Korenaga, T. and Toei, K. (1992). Fully automated system for the continuous monitoring of ammonium ion in fish farming plant sea water by flow-injection analysis. Analytica Chimica Acta 261, 345–49.Google Scholar
  17. Pilloton, R., Mignogna, G. and Fortunato, A. (1994). Entirely automated glucose monitoring system based on a flow injection analysis apparatus. Analytical Letters 27, 833–48.Google Scholar
  18. Russo, R.C. (1985) Ammonia, nitrite, and nitrate. In G.M., Rand, and S. R. Petrocelli, (eds) Fundamentals of Aquatic Toxicology, p. 455. New York: Hemisphere Publication.Google Scholar
  19. Ruzicka, J. and Hansen, E.H. (1988) Flow Injection Analysis, 2nd ed. New York: Wiley.Google Scholar
  20. Sharf, B.W. (1979). A fish test alarm device for the continual recording of acute toxic substances in water. Archive für Hydrobiologie 85, 250–6.Google Scholar
  21. Styrishave, B., Rasmussen, A.D. and Depledge, M.H. (1995). The influence of bulk and trace metals on the circadian rhythm in fresh water crayfish (Astacus astacus). Marine Pollution Bulletin 31, 87–92.Google Scholar
  22. Tonapi, G.T. and Varghese, G. (1984). Cardiophysiological responses of the crab Berytelphusa cunnicularis to three common pollutants. Indian Journal of Experimental Biology 22, 548–9.Google Scholar
  23. Tonapi, G.T. and Varghese, G. (1987). Cardio-physiological responses of some selected cladocerans to three common pollutants. Archive für Hydrobiologie 110, 59–65.Google Scholar
  24. Van Hoof, F. (1980). Evaluation of an automatic system for detection of toxic substances in surface water using trout. Bulletin of Environmental Contamination and Toxicology 25, 221–5.Google Scholar
  25. Worsfold, P.J., Clinch, J.R. and Casey, H. (1987). Spectrophotometric field monitor for water quality parameters. Determination of phosphate. Analytica Chimica Acta 197, 43–50.Google Scholar
  26. Worsfold, P.J. (1994). Environmental monitoring—a flow injection approach. Journal of Automatic Chemistry 16, 153–4.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • M. J. Bloxham
    • 1
  • P. J. Worsfold
    • 1
  • M. H. Depledge
    • 1
  1. 1.Plymouth Environmental Research CentreUniversity of PlymouthDevonUnited Kingdom

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