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Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Interacts with Human Myeloid Cell Nuclear Differentiation Antigen Induced by Interferon α

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Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpes virus type 8 (HHV-8) is tightly linked to the development of Kaposi's sarcoma, primary effusion lymphoma (PEL) and some cases of multicentric Castleman's disease. Latency-associated nuclear antigen (LANA) is one of a limited number of KSHV genes consistently expressed in these diseases as well as in KSHV-infected cell lines derived from PEL, and has been shown to play crucial role in persistence of KSHV genomes in the infected cells. In this study, we explored the cellular factors that interact with LANA using yeast two-hybrid screening, and isolated a part of gene encoding human myeloid cell nuclear differentiation antigen (MNDA). MNDA is a hematopoietic interferon-inducible nuclear proteins with a HIN-200 family member with conserved 200-amino acid repeats. Immunoprecipitation assay revealed that LANA interacted with MNDA in a mammalian embryonic kidney cell line. MNDA transcript was undetectable in three PEL cell lines by reverse-transcription polymerase chain reaction, but it was induced by interferon α (IFNα). Moreover, LANA and MNDA were co-localized in the nuclei of MNDA-expressing PEL cells. Our results suggest that LANA interacts with MNDA in KSHV-infected cells exposed to IFNα. Such interaction may modulate IFN-mediated host defense activities.

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Authors and Affiliations

  1. Division of Virology, Niigata University Graduate School of Medical and Dental Sciences, 1-757, Asahimachi-Dori, Niigata, 951-8510, Japan

    Masaya Fukushi, Masaya Higuchi, Masayasu Oie, Takafumi Tetsuka & Masahiro Fujii

  2. Division of Clinical Nephrology, Rheumatology, Respiratory Medicine and Infection Control and Prevention, Niigata University Graduate School of Medical and Dental Sciences, 1-757, Asahimachi-Dori, Niigata, 951-8510, Japan

    Takafumi Tetsuka

  3. Virology Laboratory, Department Pathology and Microbiology, University Teaching Hospital, P/Bag RW 1X, Lusaka, Zambia

    Francis Kasolo & Kouji Ichiyama

  4. Department of Molecular Virology, Division of Infection and Bioresponse Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Kouji Ichiyama & Naoki Yamamoto

  5. Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku-ku, Tokyo, 162-8640, Japan

    Harutaka Katano & Tetsutaro Sata

Authors
  1. Masaya Fukushi
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  2. Masaya Higuchi
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  3. Masayasu Oie
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  4. Takafumi Tetsuka
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  5. Francis Kasolo
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  6. Kouji Ichiyama
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  7. Naoki Yamamoto
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  8. Harutaka Katano
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  9. Tetsutaro Sata
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  10. Masahiro Fujii
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Corresponding author

Correspondence to Masahiro Fujii.

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Fukushi, M., Higuchi, M., Oie, M. et al. Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Interacts with Human Myeloid Cell Nuclear Differentiation Antigen Induced by Interferon α. Virus Genes 27, 237–247 (2003). https://doi.org/10.1023/A:1026391715071

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  • DOI: https://doi.org/10.1023/A:1026391715071

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