Abstract
The partial amino acid sequences of 121 rice proteins separated by two-dimensional gel electrophoresis (2D-PAGE), were determined for a protein sequence data file. In the Rice Genome Research Program (RGP), more than 20,000 cDNA clones randomly selected from rice cDNA libraries have been sequenced to construct a cDNA catalog. Complimentary DNAs encoding about 30% of proteins in the protein sequence data file could be identified in the catalog by computer search. It was deduced that 20,000–40,000 genes are present in the rice genome. Only half of about 20,000 cDNAs sequenced in the RGP, corresponding to 1/4–1/2 of genes present in the entire rice genome, should have unique sequences after considering gene redundancy. This is consistent with the fact that the cDNAs encoding about 30% of the sequenced proteins could be identified in the catalog. If the size of the cDNA catalog is enlarged further, cDNAs encoding all proteins separated by 2D-PAGE could be easily identified from the catalog by using the protein sequence data.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Cleveland, D. W., Fischer, S. G., Kirschner, M. W., and Laemmli, U. K. (1977). J. Biol. Chem. 252, 1102–1106.
Hirano, H. (1982). Phytochemistry 21, 1531–1518.
Hirano, H., and Watanabe, T. (1990). Electrophoresis 11, 573–580.
Hirano, H. (1989). J. Protein Chem. 8, 115–130.
Hirano, H., Komatsu, S., Nakamura, A., Kikuchi, F., Kajiwara, H., Tsunasawa, S., and Sakiyama, F. (1991). Theor. Appl. Genet. 83, 153–158.
Hirano, H., Komatsu, S., Takakura, H., Sakiyama, F., and Tsunasawa, S. (1992). J. Biochem. 111, 754–757.
Hirano, H., Komatsu, S., Kajiwara, H., Takagi, Y., and Tsunasawa, S. (1993). Electrophoresis 14, 839–846.
Komatsu, S., Kajiwara, H., and Hirano, H. (1993). Theor. Appl. Genet. 86, 935–942.
O'Farrell, P. H. (1975). J. Biol. Chem. 250, 4007–4021.
Pearson, W. R., and Lipman, D. J. (1988). Proc. Natl. Acad. Sci. USA 85, 2444–2448.
Sasaki, T., Song, J., Koga-Ban, Y., Matsui, E., Fang, F., Higo, H., Nagasaki, H., Miya, M., Murayama-Kayano, E., Takiguchi, T., Takasuga, A., Niki, T., Ishimaru, K., Ikeda, H., Yamamoto, Y., Mukai, Y., Ohta, I., Miyadera, N., Havukkala, I., and Minobe, Y. (1994). Plant J. 6, 615–624.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirano, H. Screening of Rice Genes from the cDNA Catalog Using the Data Obtained by Protein Sequencing. J Protein Chem 16, 533–536 (1997). https://doi.org/10.1023/A:1026377931210
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1026377931210