Abstract
1. The growth inhibitory factor (GIF) is a 68-amino acid protein which is capable of inhibiting the growth of neuronal cells in vitro.
2. We have cloned and sequenced the 5′-flanking region of the mouse GIF gene, which spans from the transcriptional initiation site to the −1854 nucleotide.
3.This region contains sequences homologous to hgcs, SPE, and the JCV silencer domain that functions in a glial cell specific manner. This region also contains two metal responding elements and putative binding sites for AP-1, AP-2, Sp-1, SP-2, NF-1, and CREB.
4. An analysis of the reporter plasmids containing the various regions of the 5′-flanking sequence revealed that the region indeed functioned in a tissue-specific manner in glial cells and that the region between −328 and 175 is responsible for suppression, while the region between −175 and −49 is involved in the activation of gene expression.
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Watabe, M., Gross, S., Lawyer, C. et al. Sequence and Functional Analysis of the 5′-Flanking Region of the Mouse Growth Inhibitory Factor Gene. Cell Mol Neurobiol 17, 235–243 (1997). https://doi.org/10.1023/A:1026369913405
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DOI: https://doi.org/10.1023/A:1026369913405