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Cloning and characterization of the human IFT20 gene*

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Abstract

Intraflagellar transport (IFT), in which molecular motors and IFT particle proteins participate, is very important in assembling and maintaining many cilia/flagella, such as the motile cilia that drive the swimming of cells and embryos, the nodal cilia that generate left-right asymmetry in vertebrate embryos, and the sensory cilia that detect sensory stimuli in some animals. Here we report the molecular cloning and characterization of a novel human cDNA that is 1060 base pair in length, encoding a putative protein of 158 amino acid residues which shares high homology to Mus musculus intraflagellar transport protein mIFT20 (Accession number AAL99202, identity 82.3%, similarity 82.9%) at protein level. Bioinformatics analysis revealed that the gene is composed of 6 exons and is located on human chromosome 17p11.1. RT-PCR showed that this gene is expressed in human brain, lung, kidney and pancreas, and lower expression were also detected in human placenta, liver, thymus, prostate and testis.

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Authors and Affiliations

  1. State key laboratory of genetic engineering, Institute of Genetics, School of life sciences, Fudan University, Shanghai, 200433, P.R. China

    Gang Yin, JianLiang Dai, Chaoneng Ji, Xiaohua Ni, Guang Shu, Xin Ye, Jianfeng Dai, Qihan Wu, Shaohua Gu, Yi Xie & Yumin Mao

  2. Chinese Academy of Medical Science & Peking Union Medical College, Center, National Key Laboratory of Experimental Hematology, Beijing, P.R, China

    Robert Chunhua Zhao

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  1. Gang Yin
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  2. JianLiang Dai
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  3. Chaoneng Ji
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  4. Xiaohua Ni
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  5. Guang Shu
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  6. Xin Ye
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  7. Jianfeng Dai
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  8. Qihan Wu
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  9. Shaohua Gu
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  10. Yi Xie
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  11. Robert Chunhua Zhao
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Yin, G., Dai, J., Ji, C. et al. Cloning and characterization of the human IFT20 gene* . Mol Biol Rep 30, 255–260 (2003). https://doi.org/10.1023/A:1026365124176

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  • DOI: https://doi.org/10.1023/A:1026365124176

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