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Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa

Fish Physiology and Biochemistry volume 26, pages 289–295 (2002)Cite this article

Abstract

This paper provides data concerning biochemical composition of milt of two sturgeon species, Siberian sturgeon bred in aquaculture facility of Inland Fisheries Institute in North Poland and sterlet (from two different populations from Danube and Odra). Milt plasma of Siberian sturgeon and sterlet, when compared to teleost fish, is characterized by much lower osmolality (up 70 to 96 mOsm kg−1) and protein concentration (0.24–0.58 g l−1). In spite of the presence of an acrosome and acrosin the antiproteinase activity of seminal plasma was low (12.79–25.40 U l−1). Activities of arylsulfatase and β-N-acetylglucosaminidase were found in spermatozoa. This agrees with the presence of an acrosome in sturgeons sperm. Similarly to mammals, these enzymes are also present in milt plasma. We determined a range of enzymatic activities from the minimal (seminal plasma) to the maximal damage (supernatants obtained after freezing-thawing without cryoprotectant). Activities of arylsulfatase, β-N-acetylglucosaminidase, lactic dehydrogenase and acid phosphatase were released from spermatozoa after freezing-thawing. For this reason they are good potential candidates as a markers of cryoinjury to sperm acrosome and midpiece.

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Authors and Affiliations

  1. Molecular Andrology Group, Institute of Animal Reproduction and Food Research, 10- 747 Olsztyn, Tuwima, 10, Poland

    B. Piros & J. Glogowski

  2. Inland Fisheries Institute, 10-719 Olsztyn, Oczapowskiego 10, Poland

    R. Kolman

  3. Department of General Zoology, Szczecin University, Poland

    A. Rzemieniecki & J. Domagala

  4. Faculty of Agricultural and Envitonmental Sciences, Department of Fish Culture, Szent Istvàn Uviversity, Gödöllõ, Hungary

    Á. Horváth & B. Urbanyi

  5. Semen Biology Group, Institute of Animal Reproduction and Food Research, 10- 747 Olsztyn, Tuwima, 10, Poland

    A. Ciereszko

Authors
  1. B. Piros
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  2. J. Glogowski
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  3. R. Kolman
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  4. A. Rzemieniecki
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  5. J. Domagala
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  6. Á. Horváth
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  7. B. Urbanyi
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  8. A. Ciereszko
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Piros, B., Glogowski, J., Kolman, R. et al. Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa. Fish Physiology and Biochemistry 26, 289–295 (2002). https://doi.org/10.1023/A:1026280218957

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  • DOI: https://doi.org/10.1023/A:1026280218957

  • milt
  • seminal plasma
  • Siberian sturgeon
  • spermatozoa
  • sterlet

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