Abstract
Americium-241 is one of the most serious radioactive contaminating nuclides due to its high toxicity and long half-life. The encouraging biosorption of 241Am from aqueous solutions by free Saccharomyces cerevisiae (S. cerevisiae) has been observed in our previous experiments. In this study, the 241Am biosorption by immobilized S. cerevisiae and the effect of the various experimental conditions on the adsorption were investigated. The results indicated that the 241Am biosorption by immobilized S. cerevisiae is still very efficient, and immobilized S. cerevisiae can be used repeatedly or continuously. The biosorption equilibrium was achieved within 2 hours, and more than 92% of 241Am was removed by immobilized S. cerevisiae in the pH 1-4 range. No significant differences in 241Am biosorption were observed at 15-45 °C. The immobilized S. cerevisiae, even after used repeatedly for 6 times, still could adsorb more than 90% of 241Am in solutions of 1.08 MBq/l (8.5 mg/l). At this moment, the total adsorption capacity for 241Am was more than 63.3 KBq/g globe (0.5 mg/g), but has not reached saturation yet. The 241Am left in solutions with initial concentration of 1.08 MBq/l (8.5 mg/l) was noted as low as ~10 Bq/l (~8.0'10-5 mg/l) after adsorption by the immobilized S. cerevisiae for 3 times.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
C. Z. Ma, Handbook of Radioactive Isotopes, Science Press, Beijing, 1979, p. 652.
M. Benedict, H. P. Pigford, H. W. Levi, Nuclear Chemical Engineering, 2nd ed., McGraw-Hill Book Comp., Philadelphia, PA, 1981, p. 465.
K. L. A. Nash, Solvent Extr. Ion. Exch., 11 (1993) 729.
G. A. Ye, J. Y. He, Y. Q. Jiang, J. Nucl. Radiochem., 22 (2000) 65 (in Chinese).
Y. J. Zhu, J. F. Chen, G. R. Choppin, Radiochim. Acta, 68 (1995) 95.
N. Liu, S. Z. Luo, Y. Y. Yang et al., J. Radioanal. Nucl. Chem., 252 (2002) 187.
N. Hafez, A. S. Abdel-Razek, M. M. Hafez, J. Chem. Tech. Biotechnol., 68 (1997) 19.
W. Bae, W. Chen, A. Mulchandani et al., Biotech. Bioeng., 70 (2000) 518.
J. Elmen, W. Pan, S. Y. Leung et al., Biotech. Bioeng., 55 (1997) 82.
K. F. Reardon, D. C. Mosteller, J. D. B. Rogers, Biotech. Bioeng., 69 (2000) 385.
T. Horikoshi, A. Nakajima, T. Sakauchi et al., Eur. J. Appl. Microbiol. Biotechnol., 12 (1981) 90.
A. Nakajima, T. Sakaguchi, Appl. Microbiol. Biotechnol., 24 (1986) 59.
N. Kuyucak, B. Volesky, Biotech. Lett., 10 (1988) 137.
Z. R. Holan, B. Volesky, I. Prasetyo et al., Biotech. Bioeng., 41 (1993) 819.
M. E. Mullen, D. C. Wolf, F. G. Ferris, Appl. Environ. Microbiol., 12 (1989) 3143.
M. Tsezos, D. M. Keller, Biotech. Bioeng., 25 (1983) 201.
N. Friis, P. Myers-Keith, Biotech. Bioeng., 28 (1986) 21.
A. Nakajima, T. Horikoshi, T. Sakauchi, Eur. J. Appl. Microbiol. Biotechnol., 16 (1982) 88.
G. M. Gadd, C. White, Biotech. Bioeng., 33 (1988) 592.
M. Tsezos, R. G. L. McCready, J. P. Bell, Biotech. Bioeng., 34 (1988) 10.
C. White, G. M. Gadd, J. Chem. Tech. Biotechnol., 49 (1990) 331.
J. R. Lloyd, L. Harding, L. E. Macaskie, Biotech. Bioeng., 55 (1997) 505.
N. P. Barkley, J. Air Waste Managem. Assoc., 41 (1991) 1381.
C. White, G. M. Gadd, J. General Microbiol., 133 (1987) 727.
B. Volesky, H. A. May-Philips, Biotechnol. Bioeng., 41 (1993) 826.
D. Brady, J. R. Duncan, Enzym. Microbiol. Technol., 16 (1994) 633.
J. R. Zhu, L. Xu, Chin. J. Appl. Environ. Biol., 4 (1998) 400.
B. Volesky, H. A. May-Philips, Appl. Microbiol. Biotechnol., 42 (1995) 797.
L. W. Yang, Appl. Microorg., 2 (1984) 13 (in Chinese).
S. L. Xia, Experimental Techniques of Advanced Applied Microorganism, Publishing House of Light Industry, Beijing, 1988.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, N., Liao, J., Luo, S. et al. Biosorption of 241Am by immobilized Saccharomyces cerevisiae . Journal of Radioanalytical and Nuclear Chemistry 258, 59–63 (2003). https://doi.org/10.1023/A:1026249823849
Issue Date:
DOI: https://doi.org/10.1023/A:1026249823849