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cDNA Microarray Analysis of Changes in Gene Expression Associated with MPP+ Toxicity in SH-SY5Y Cells

Neurochemical Research volume 28, pages 1873–1881 (2003)Cite this article

Abstract

cDNA microarray analysis of 1-methyl-4-phenyl-pyridinium (MPP+) toxicity (1 mM, 72 h) in undifferentiated SH-SY5Y cells identified 48 genes that displayed a signal intensity greater than the mean of all differentially expressed genes and a two-fold or greater difference in normalized expression. RT-PCR analysis of a subset of genes showed that c-Myc and RNA-binding protein 3 (RMB3) expression decreased by ∼50% after 72 h of exposure to MPP+ (1 mM) but did not change after 72 h of exposure to 6-hydroxydopamine (25 μM), rotenone (50 nM), and hydrogen peroxide (600 μM). Exposure of retinoic acid (RA)-differentiated SH-SY5Y cells to MPP+ (1 mM, 72 h) also resulted in a decrease in RMB3 expression and an increase in GADD153 expression. In contrast, c-Myc expression was slightly increased in RA-differentiated cells. Collectively, these data provide new insights into the molecular mechanisms of MPP+ toxicity and show that MPP+ can elicit distinct patterns of gene expression in undifferentiated and RA-differentiated SH-SY5Y cells.

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Affiliations

  1. Department of Veterans Affairs, VA Medical Center, 200 Springs Road, Bedford, Massachusetts

    Kelly J. Conn, M. David Ullman, Michelle J. Larned, Patricia B. Eisenhauer, Richard E. Fine & John M. Wells

  2. Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts

    Kelly J. Conn

  3. Department of Psychiatry, Boston University School of Medicine, Boston, Massachusett

    M. David Ullman

  4. University of Massachusetts Medical School, Waltham, Massachusetts

    M. David Ullman & Patricia B. Eisenhauer

  5. Department of Neurology, Boston University School of Medicine, Boston, Massachusetts

    Richard E. Fine & John M. Wells

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  1. Kelly J. Conn
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Correspondence to Kelly J. Conn.

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Conn, K.J., Ullman, M.D., Larned, M.J. et al. cDNA Microarray Analysis of Changes in Gene Expression Associated with MPP+ Toxicity in SH-SY5Y Cells. Neurochem Res 28, 1873–1881 (2003). https://doi.org/10.1023/A:1026179926780

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  • DOI: https://doi.org/10.1023/A:1026179926780

  • cDNA microarray
  • Parkinson's disease
  • 1-methyl-4-phenyl-pyridinium
  • gene expression
  • neurodegeneration