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Detection of p53 Gene Mutations by Nonisotopic RNase Cleavage Assay as a Predictor of Poor Prognosis in Colorectal Cancers

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Abstract

The present study was undertaken to evaluate p53 gene mutation as a prognostic factor in patients with colorectal cancer. Nonisotopic RNase cleavage assay (NIRCA), recently used for detecting gene mutations, was employed to detect p53 gene mutations in this study. In 15 samples of colorectal tumors, NIRCA was confirmed to be simple, accurate, and thus useful for clinical use, compared with polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP). In another group of 79 cases of colorectal cancer analyzed for p53 gene mutation by using NIRCA, mutations were detected in 58 of 79 (73.4%) cases. Multivariate Cox proportional-hazards analysis showed that p53 gene mutation was a significant prognostic factor in patients with colorectal cancer. Our results showed that NIRCA is a simple and sensitive method, and thus useful for genetic screening of colorectal cancer. Furthermore, our results showed that p53 gene mutation is an independent predictor of poor prognosis in colorectal cancers.

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Authors and Affiliations

  1. First Department of Surgery, Nagasaki University School of Medicine, Japan

    Masatoshi Haseba, Shigekazu Hidaka, Takashi Tsuji, Hiroshi Yano, Hideaki Komatsu, Terumitu Sawai, Toru Yasutake, Tohru Nakagoe & Hiroyoshi Ayabe

  2. Nagasaki School of Allied Medical Sciences, Nagasaki University, Nagasaki, Japan

    Yutaka Tagawa

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  1. Masatoshi Haseba
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  2. Shigekazu Hidaka
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  3. Takashi Tsuji
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  4. Hiroshi Yano
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  5. Hideaki Komatsu
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  7. Toru Yasutake
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  9. Yutaka Tagawa
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  10. Hiroyoshi Ayabe
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Correspondence to Masatoshi Haseba.

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Haseba, M., Hidaka, S., Tsuji, T. et al. Detection of p53 Gene Mutations by Nonisotopic RNase Cleavage Assay as a Predictor of Poor Prognosis in Colorectal Cancers. Dig Dis Sci 48, 1984–1989 (2003). https://doi.org/10.1023/A:1026126522278

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