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Host-specific plant signal and G-protein activator, mastoparan, trigger differentiation of zoospores of the phytopathogenic oomycete Aphanomyces cochlioides

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Abstract

We found that the gradient of a host-specific attractant, cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone) isolated from the roots of spinach triggered encystment followed by germination of zoospores of Aphanomyces cochlioidesat a concentration less than micromolar order. This compound did not affect the growth and reproduction of this phytopathogen up to 10−6 M concentration in the culture medium. We also observed that mastoparan, an activator of heterotrimeric G-protein could inhibit the motility of zoospores and then strikingly effect encystment followed by 60–80% germination of cysts. Concomitant application of cochliophilin A and mastoparan showed stronger encystment followed by 100% germination of cysts. In addition, we have observed that chemicals interfering with phospholipase C activity (neomycin) and Ca2+ influx/release (EGTA and loperamide) suppress cochliophilin A or mastoparan induced encystment and germination. These results suggest that G-protein mediated signal transduction mechanism may be involved in the differentiation of the A. cochlioides zoospores. This is the first report on the differentiation of oomycete zoospores initiated by a host-specific plant signal or a G-protein activator.

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Author notes

  1. Md. Tofazzal Islam

    Present address: School of Agriculture and Rural Development, Bangladesh Open University, Gazipur-, 1705, Bangladesh

Authors and Affiliations

  1. Laboratory of Ecological Chemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, 060-8589, Japan

    Md. Tofazzal Islam & Satoshi Tahara

  2. Laboratory of Electronic Microscopy, Faculty of Agriculture, Hokkaido University, Japan

    Toshiaki Ito

Authors
  1. Md. Tofazzal Islam
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  2. Toshiaki Ito
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  3. Satoshi Tahara
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Correspondence to Satoshi Tahara.

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Islam, M.T., Ito, T. & Tahara, S. Host-specific plant signal and G-protein activator, mastoparan, trigger differentiation of zoospores of the phytopathogenic oomycete Aphanomyces cochlioides . Plant and Soil 255, 131–142 (2003). https://doi.org/10.1023/A:1026114731718

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