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Identification of resistance gene analogs in cotton (Gossypium hirsutum L.)

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Abstract

Sequence analyses of numerous plant disease resistance genes have revealed the presence of conserved motifs common to this class of genes, namely a nucleotide binding site (NBS) and leucine rich repeat region. In this study, thirty-three resistance gene analogs (RGAs) were cloned and sequenced from cotton (Gossypium hirsutum L.) following PCR with degenerate primers designed from the conserved NBS motif of plant resistance (R) genes. Phylogenetic analysis of the predicted amino acid sequences grouped the RGAs into four distinct classes from which several subgroups were delineated based on nucleic acid sequences. Gene database searches with the consensus protein sequences of each of the four classes and respective subgroups of cotton RGAs revealed their conserved NBS domains and homology to RGAs and known resistance genes from a variety of plant genera. Given the complete lack of knowledge regarding molecular organization of R genes in cotton, the cloned RGAs described here may be useful as probes to map, characterize, and manipulate R genes of the cotton genome.

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Authors and Affiliations

  1. Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, MS, 39762, U.S.A

    H. Tan & R.G. Creech

  2. USDA-ARS, Crop Science Research Laboratory, Genetics and Precision Agriculture Research Unit, Mississippi State, MS, 39762, U.S.A.

    F.E. Callahan, S. Saha & J.N. Jenkins

  3. Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS, 39762, U.S.A

    X.-D. Zhang, M. Karaca & D.-P. Ma

Authors
  1. H. Tan
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  2. F.E. Callahan
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  3. X.-D. Zhang
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  4. M. Karaca
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  5. S. Saha
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  6. J.N. Jenkins
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  7. R.G. Creech
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  8. D.-P. Ma
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Tan, H., Callahan, F., Zhang, XD. et al. Identification of resistance gene analogs in cotton (Gossypium hirsutum L.). Euphytica 134, 1–7 (2003). https://doi.org/10.1023/A:1026114327168

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