Skip to main content
SpringerLink
Log in

Molecular mapping of a new gene Rrs4 CI 11549 for resistance to barley scald (Rhynchosporium secalis)

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Doubled haploid (DH) progeny from a cross between the scald susceptible barley (Hordeum vulgare L.) cultivar ‘Ingrid’ and the resistant accession ‘CI 11549’ (‘Nigrinudum’) was evaluated for resistance in the pathogen Rhynchosporium secalis (Oudem) J.J. Davis. Two linked and incompletely dominant loci confer resistance ‘CI 11549’ against isolate ‘4004’. One is an allele at the complex Rrs1 locus on chromosome 3H close to the centromere; the other is located 22 cM distally on the long arm. The latter locus is designated Rrs4. In BC3-lines into ‘Ingrid’ from ‘CI 2222’ (another ‘Nigrinudum’) resistance seems governed by one locus close to the telomeric region of chromosome 7H, probably allelic to Rrs2. In neither case did we find any trace of the recessive gene rh8 reported to be present in ‘Nigrinudum’. Various resistance donors of Ethiopian origin designated as ‘Nigrinudum’, ‘Jet’ or ‘Abyssinian’ were identical to a great extent with respect to markers, but differed in resistance to different isolates of scald or in barley yellow dwarf virus (BYDV) resistance. The implications for their use as differentials in scald tests and screening of germplasm collections are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abbott D.C., Brown A.H.D. and Burdon J.J. 1992. Genes for scald resistance from wild barley (Hordeum vulgare ssp spontaneum) and their linkage to isozyme markers. Euphytica 61: 225–231.

    Google Scholar 

  • Abbott D.C., Lagudah E.S. and Brown A.H.D. 1995. Identification of RFLPs flanking a scald resistance gene on barley chromosome 6. J. Hered. 86: 152–154.

    Google Scholar 

  • Alemayehu F. and Parlevliet J.E. 1997. Variation between and within Ethiopian barley landraces. Euphytica 94: 183–189.

    Google Scholar 

  • Asíns M.J. 2002. Present and future of quantitative trait locus analysis in plant breeding. Plant Breed. 121: 281–291.

    Google Scholar 

  • Baker R.J. and Larter E.N. 1963. The inheritance of scald resistance in barley. Can. J. Genet. Cytol. 5: 445–449.

    Google Scholar 

  • Barua U.M., Chalmers K.J., Hacket C.A., Thomas W.T.B., Powell W. and Waugh R. 1993. Identification of RAPD markers linked to Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71: 177–184.

    Google Scholar 

  • Becker J. and Heun M. 1995. Barley microsatellites: allele variation and mapping. Plant. Mol. Biol. 27: 835–845.

    Google Scholar 

  • Bjørnstad Å., Skinnes H. and Thoresen K. 1993. Comparisons between doubled haploid lines produced by anther culture, for Hordeum bulbosum-method and lines produced by single seed descent. Euphytica 66: 135–144.

    Google Scholar 

  • Bjørnstad Å., Demissie A., Kilian A. and Kleinhofs A. 1997. The distinctness and diversity of Ethiopian barley. Theor. Appl. Genet. 94: 514–521.

    Google Scholar 

  • Bjørnstad Å, Patil V., Tekauz A., Marøy A.G., Skinnes H., Jensen A. et al. 2002. Resistance to scald (Rhynchosporium secalis) in barley (Hordeum vulgare L.) studied by near-isogenic lines. I. Markers and differential isolates. Phytopathology 92: 710–720.

    Google Scholar 

  • Bockelmann H.E., Sharp E.L. and Eslick R.F. 1977. Trisomic analysis of genes for resistance to scald and net blotch in several barley cultivars. Can. J. Bot. 55: 2142–2148.

    Google Scholar 

  • Bockelmann H.E., Eslick R.F. and Sharp E.L. 1980. Registration of the barley composite cross XXXVI. Crop. Sci. 20: 575–576.

    Google Scholar 

  • Brown J.S. 1985. Pathogenic variations among isolates of Rhynchosporium secalis from cultivated barley growing in Victoria, Australia. Euphytica 34: 129–133.

    Google Scholar 

  • Ceoloni C. 1980. Race differentiation and search for sources of resistance to Rhynchosporium secalis in barley in Italy. Euphytica 29: 547–553.

    Google Scholar 

  • Churchill G.A. and Doerge R.W. 1994. Empirical threshold values for quantitive trait mapping. Genetics 138: 963–971.

    Google Scholar 

  • Demissie A. and Bjørnstad Å. 1996. Phenotypic diversity of Ethiopian barleys in relation to geographical regions, altitudinal range, and agro-ecological zones: as an aid to germplasm collection and conservation strategy. Hereditas 124: 17–29.

    Google Scholar 

  • Devaux P., Kilian A. and Kleinhofs A. 1995. Comparative mapping of the barley genome with male and female recombination-derived, doubled haploid populations. Mol. Gen. Genet. 249: 600–608.

    Google Scholar 

  • Dyck P.L. and Schaller C.W. 1961a. Inheritance in barley of resistance to several physiologic races of the scald fungus. Can. J. Genet. Cytol. 3: 153–164.

    Google Scholar 

  • Dyck P.L. and Schaller C.W. 1961b. Association of two genes for scald resistance with specific barley chromosome. Can. J. Genet. Cytol. 3: 165–169.

    Google Scholar 

  • Engels J.M.M. 1994. Genetic diversity in Ethiopian barley in relation to altitude. Genetic Resources Crop. Evol. 41: 67–73.

    Google Scholar 

  • Ford C.M., Paltridge N.G., Moritz R.L., Simpson R.J. and Symons R.H. 1998. Rapid and informative assays for Yd2, the barley yellow dwarf virus resistance gene, based on the nucleotide sequence of a closely linked gene. Mol. Breed. 4: 23–31.

    Google Scholar 

  • Garvin D.F., Brown A.H.D. and Burdon J.J. 1997. Inheritance and chromosome locations of scald-resistance genes derived from Iranian and Turkish wild barleys. Theor. Appl. Genet. 94: 1086–1091.

    Google Scholar 

  • Garvin D.F., Brown A.H.D., Raman H. and Read B.J. 2000. Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers. Plant Breed. 119: 193–196.

    Google Scholar 

  • Gilbert J.E., Lewis R.V., Wilkinson M.J. and Caligari P.D.S. 1999. Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor. Appl. Genet. 98: 1125–1131.

    Google Scholar 

  • Goodwin S.B., Webster P.K. and Allard R.W. 1994. Evidence for mutation and migration as sources of genetic variation in populations of Rhynchosporium secalis. Phytopathology 84: 1047–1053.

    Google Scholar 

  • Graner A. and Tekauz A. 1996. RFLP mapping in barley of a dominant gene conferring resistance to scald (Rhynchosporium secalis). Theor. Appl. Genet. 93: 421–425.

    Google Scholar 

  • Graner A., Jahoor A., Schondelmaier J., Siedler H., Pillen K., Fischbeck G. et al. 1991. Construction of an RFLP map of barley. Theor. Appl. Genet. 83: 250–256.

    Google Scholar 

  • Grønnerød S., Bjørnstad Å., Marøy A.G., MacKey J., Tekauz A. and Penner G.A. 2002. Genetic analysis of resistance to barley scald (Rhynchosporium secalis). I. The Ethiopian line ‘Abyssinian’ (CI 668). Euphytica 126: 235–250.

    Google Scholar 

  • Habgood R.M. and Hayes J.D. 1971. The inheritance of resistance to Rhynchosporium secalis in barley. Heredity 27: 25–37.

    Google Scholar 

  • Heun M., Kennedy A.E., Anderson J.E., Lapitan N.L.V., Sorrels M.E. and Tanksley S.D. 1991. Construction of a restriction fragment length polymorphism map of barley (Hordeum vulgare). Genome. 34: 437–447.

    Google Scholar 

  • Jensen C.J. 1976. Barley monoploids and doubled monoploids: techniques and experience. Barley Genet. 3: 316–345.

    Google Scholar 

  • Jørgensen H.J.L. and Smedegaard-Petersen V. 1995. Pathogenic variation of Rhynchosporium secalis in Denmark and sources of resistance in barley. Plant Dis. 79: 297–301.

    Google Scholar 

  • Kleinhofs A., Kilian A., Saghai Maroof M.A., Biyashev R.M., Hayes P., Chen F.Q. et al. 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86: 705–712.

    Google Scholar 

  • Kosambi D.D. 1944. The estimation of map distances from recombinant values. Ann. Eugen. 12: 172–175.

    Google Scholar 

  • Liu Z.W., Biyashev R.M. and Saghai Maroof M.A. 1996. Development of simple sequence repeat DNA markers and their integration into barley linkage map. Theor. Appl. Genet. 93: 869–876.

    Google Scholar 

  • Ooijen van J.W. 1994. A computer program for drawing genetic linkage maps. J. Hered. 85: 66.

    Google Scholar 

  • Paltridge N.G., Collins N.C., Bendahmane A. and Symons R.H. 1998. Development of YLM, a codominant PCR marker closely linked to Yd2 gene for the resistance to barley yellow dwarf disease. Theor. Appl. Genet. 96: 1170–1177.

    Google Scholar 

  • Patil V., Bjørnstad Å., Magnus H. and Mac Key J. 2002. Resistance to scald (Rhynchosporium secalis) in barley (Hordeum vulgare L.). II. Diallele analysis of near-isogenic lines. Hereditas 137: 186–197.

    Google Scholar 

  • Penner G.A., Tekauz A., Reimer E., Scoles G.J., Rossnagel G.G., Eckstein P.E. et al. 1996. The genetic basis of scald resistance in western Canadian barley cultivars. Euphytica 92: 367–374.

    Google Scholar 

  • Ramsay L., Macaulay M., McLean K., Fuller J., Edwards K., Tuvesson S. et al. 2000. A simple sequence repeat - based linkage map of barley. Genetics 156: 1997–2005.

    Google Scholar 

  • Reitan L., Grønnerød S., Ristad T., Salamati S., Skinnes H., Waugh R. et al. 2002. Characterisation of resistance genes against scald (Rhynchosporium secalis (Oudem.) J.J. Davis) in barley (Hordeum vulgare L.) lines from central Norway, by means of genetic markers and pathotype tests. Euphytica 123: 31–39.

    Google Scholar 

  • Russell J., Fuller J., Young G., Thomas B., Taramino G., Macauly M. et al. 1997. Discriminating between barley genotypes using microsatellite markers. Genome. 40: 442–450.

    Google Scholar 

  • Saghai Maroof M.A., Biyashev R.M., Yang G.P., Zhang Q. and Allard R.W. 1994. Extraordinarily polymorphic microsatellite DNA in barley: Species diversity, chromosomal locations and population dynamics. Proc. Natl. Acad. Sci. USA 91: 5466–5470.

    Google Scholar 

  • Salamati S. and Tronsmo A.M. 1997. Pathogenicity of Rhynchosporium secalis isolates from Norway on 30 cultivars of barley. Plant Pathol. 46: 416–424.

    Google Scholar 

  • Salamati S., Zhan J., Burdon J.J. and McDonald B.A. 2000. The genetic structure of field populations of Rhynchosporium secalis from three continents suggests moderate gene flow and regular recombination. Phytopathology 90: 901–908.

    Google Scholar 

  • Schaller C.W., Qualset C.O. and Rutger J.N. 1963. Inheritance and linkage of the Yd2 gene conditioning resistance to the barley yellow dwarf virus disease in barley. Crop. Sci. 4: 544–548.

    Google Scholar 

  • Schweizer G.F., Baumer M., Daniel G., Rugel H. and Röder M.S. 1995. RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley. Theor. Appl. Genet. 90: 920–924.

    Google Scholar 

  • Stam P. and van Ooijen J.W. 1995. Joinmap™ version 2.0: Software for the Calculation of Genetic Linkage Maps. CPRODLO, Wageningen.

    Google Scholar 

  • Struss D. and Plieske J. 1998. The use of microsatellites for the detection of genetic diversity in barley populations. Theor. Appl. Genet. 97: 308–315.

    Google Scholar 

  • Søgaard B. and von Wettstein-Knowles P. 1987. Barley: Genes and Chromosomes. Carlsberg Res. Commun. 52: 123–196.

    Google Scholar 

  • Tekauz A. 1991. Pathogenic variation in Rhynchosporium secalis on barley in Canada. Can. J. Plant. Pathol. 13: 298–304.

    Google Scholar 

  • Utz H.F. and Melchinger A.E. 2000. PLABQTL: A Computer Program to Map QTL version 1.1. http://www.uni-hohenheim.de/~ipspwww/soft.html.

  • Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M. et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 23: 4407–4414.

    Google Scholar 

  • Wells S.A. and Skorporad W.P. 1963. Inheritance of reaction to Rhynchosporium secalis in barley. Can. J. Plant Sci. 43: 184–187.

    Google Scholar 

  • Wettstein-Knowless P. 1992. Cloned and mapped genes: Current status. In: Shewry P.R. (ed.), Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology. Alden Press, Oxford, pp. 73–98.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture and Crop Sciences, Agricultural University of Norway, Ås, N-1432, Norway

    Vishwanath Patil & Åsmund Bjørnstad

  2. Department of Plant Breeding Research, Swedish University of Agricultural Sciences, Uppsala, S-75007, Sweden

    James Mackey

Authors
  1. Vishwanath Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Åsmund Bjørnstad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James Mackey
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patil, V., Bjørnstad, Å. & Mackey, J. Molecular mapping of a new gene Rrs4 CI 11549 for resistance to barley scald (Rhynchosporium secalis). Molecular Breeding 12, 169–183 (2003). https://doi.org/10.1023/A:1026076511073

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026076511073

Search

Navigation