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Identification by R-banding and FISH of chromosome arms involved in Robertsonian translocations in several deer species

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Abstract

We constructed and analyzed the RBG-banded karyotype of five deer species: Chital (Axis axis), White-lipped deer (Cervus albirostris), Rusa deer (Cervus timorensis russa), Sambar deer (Cervus unicolor) and Eld's deer (Cervus eldi siamensis). Among these five species, only Eld's deer had been previously karyotyped using R-banding. In order to identify all the chromosome correspondences with cattle and precisely which chromosome arms are involved in Robertsonian translocations, we compared the karyotypes of these five species with those of the closely related and well-characterized species, cattle (Bos taurus) and Vietnamese Sika deer (Cervus nippon pseudaxis). Among these six deer species (the five above plus the Vietnamese Sika deer), we found thirteen different Robertsonian translocations involving nineteen different chromosome arms. Thirteen chromosome arms were identified by comparison of R-banding patterns only and the remaining six were either confirmed or identified by FISH-mapping of bovine or caprine probes previously localized in cattle. Finally, we observed that five of the thirteen Robertsonian translocations are shared by at least two species and that some chromosome arms are more frequently involved in Robertsonian translocations than others.

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Authors and Affiliations

  1. FRE2584 – CNRS Instabilité du génome et cancer, Pavillon Trouillet-Rossignol, 26 rue d'Ulm, 75248, Paris cedex 05, France

    A. Bonnet-Garnier

  2. 57 rue Cuvier, 75231, Paris cedex 05, France

    A. Bonnet-Garnier & F. Claro

  3. CIRAD-EMVT, Programme Ecosystèmes Naturels et Pastoraux, TA 30/F, Campus International de Baillarguet, 34398, Montpellier cedex 5, France

    S. Thévenon

  4. Laboratoire de Génétique Biochimique et Cytogénétique, INRA-CRJ, Domaine de Vilvert, Jouy-en-Josas, 78352, cedex, France

    M. Gautier & H. Hayes

Authors
  1. A. Bonnet-Garnier
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  2. F. Claro
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  3. S. Thévenon
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  4. M. Gautier
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  5. H. Hayes
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Bonnet-Garnier, A., Claro, F., Thévenon, S. et al. Identification by R-banding and FISH of chromosome arms involved in Robertsonian translocations in several deer species. Chromosome Res 11, 649–663 (2003). https://doi.org/10.1023/A:1025981508867

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  • DOI: https://doi.org/10.1023/A:1025981508867

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