Abstract
A rapid and efficient regeneration system has been developed for interspecific self-incompatible hybrids between Antirrhinum majus×A. hispanicum. Multiple shoots were induced from hypocotyls explants on Murashige and Skoog medium or Loblolly pine medium supplemented with a low concentration of 3-indoleacetic acid (0.05 mg l−1) and a high concentration of 6-benzyladenine (1 mg l−1). Rooting took place in 1/2 Murashige and Skoog medium without growth regulators. By using this procedure, it takes about 5–6 weeks from seed to plantlet. The hypocotyls also had a potential to expand and form callus, providing a source for a continuous supply of multiple shoots from the calli at periodic intervals.
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Wang, H., Ouyang, J. & Xue, Y. A facile procedure for efficient plantlet regeneration from self-incompatible hybrids in Antirrhinum . Plant Cell, Tissue and Organ Culture 76, 61–65 (2004). https://doi.org/10.1023/A:1025899532273
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DOI: https://doi.org/10.1023/A:1025899532273