Abstract
Leaf explants of Paphiopedilum phiIippinense hybrids (hybrid PH59 and PH60) directly formed adventitious shoots from wound regions within 1 month, when cultured on modified Murashige and Skoog medium (1/2-strength macro- and full-strength micro-elements) free of plant growth regulator in darkness. The combinations of 2,4-dichlorophenoxyacetic acid ((2,4-D) acid (0, 4.52 and 45.25 μM) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) (0, 0.45, 4.54 and 22.71 μM) were used to test their effects on direct shoot bud formation from two types of explants (1.5-cm long intact leaf explants and 0.5-cm long leaf segment explants). In hybrid PH59, 4.54 μM TDZ increased mean numbers of shoots per explant with leaf segment explants. In hybrid PH60, 4.52 μM 2,4-D plus 0.45 μM TDZ promoted direct shoot bud formation from leaf segment explants. In addition, three treatments (4.52 μM 2,4-D, 22.71 μM TDZ, 4.52 μM 2,4-D plus 4.54 μM TDZ) gave a higher response than control on mean numbers of shoots per explant with intact leaf explants. Healthy plantlets each with one to three roots were obtained from leaf-derived shoots after transfer onto a hormone-free medium for 22 months. These plantlets were acclimatized in a greenhouse and grew well with 100% survival rate.
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Chen, TY., Chen, JT. & Chang, WC. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell, Tissue and Organ Culture 76, 11–15 (2004). https://doi.org/10.1023/A:1025858211320
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DOI: https://doi.org/10.1023/A:1025858211320