Abstract
Chromatophores and peripheral light-harvesting complexes B800–850 with a trace of carotenoids were isolated from Chromatium minutissimum cells in which carotenoid biosynthesis was inhibited by diphenylamine. Three methods previously used for the reconstitution of carotenoids into either the light-harvesting (LH1) type complexes or reaction centers (RC) of carotenoidless mutants were examined for the possibility of carotenoid reconstitution into the carotenoid depleted chromatophores. All these methods were found to be unsuitable because carotenoid depleted complex B800–850 from Chr. minutissimum is characterized by high lability. We have developed a novel method maintaining the native structure of the complexes and allowing reconstitution of up to 80% of the carotenoids as compared to the control. The reconstituted complex has a similar CD spectrum in the carotenoid region as the control, and its structure restores its stability. These data give direct proof for the structural role of carotenoids in bacterial photosynthesis.
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REFERENCES
Zuber, H., and Cogdell, R. J. (1995) in Anoxygenic Photosynthetic Bacteria (Blankenship, R., Madigan, M. T., and Bauer, C. E., eds.) Kluwer Academic Publisher, Dordrecht, pp. 315-348.
Van Grondelle, R., Dekker, J. P., Gilbro, T., and Sundstrom, V. (1994) Biochim. Biophys. Acta, 1187, 1-65.
Moskalenko, A. A., and Erokhin, Yu. E. (1974) Mikrobiologiya, 43, 654-655.
Drews, G. (1985) Microbiol. Rev., 49, 59-70.
McDemott, G., Prince, S. M., Freer, A. A., Hawthornthwaite-Lawless, A. M., Papiz, M. Z., Cogdell, R. J., and Isaacs, N. W. (1995) Nature, 374, 517-521.
Koepke, J., Hu, X., Muenke, C., Schulten, K., and Michel, H. (1996) Structure, 4, 581-597.
Karrasch, S., Bullough, P. A., and Ghosh, R. (1995) EMBO J., 14, 631-638.
Picorel, R., Holt, R. E., Cotton, T., and Seibert, M. (1988) J. Biol. Chem., 263, 4374-4380.
Fowler, G. J. S., Visschers, R. W., Grief, G. G., van Grondelle, R., and Hunter, C. N. (1992) Nature, 355, 848-850.
Gall, A., Fowler, G. J. S., Hunter, C. N., and Robert, B. (1997) Biochemistry, 36, 16282-16287.
Bandilla, M., Ucker, B., Fam, M., Simonin, I., Gelhaye, E., McDermott, G., Cogdell, R. J., and Scheer, H. (1998) Biochim. Biophys. Acta, 1364, 390-402.
Grithiths, M., Sistrom, W. R., Cohen-Basire, G., and Stanier, R. Y. (1955) Nature, 176, 1211-1214.
Giuliano, G., Pollock, D., Stapp, H., and Scolnik, P. (1988) Mol. Gen. Genet., 213, 78-83.
Lang, H., Cogdell, R. J., Takachi, S., and Hunter, C. N. (1995) J. Bacteriol., 177, 2064-2073.
Brunisholz, R. A., Zuber, H., Valentine, J., Lindsay, J. G., Wooley, K. J., and Cogdell, R. J. (1986) Biochim. Biophys. Acta, 849, 295-303.
Jones, M. R., Fowler, G. J. S., Gibson, L. C. D., Grief, G. G., Olsen, J. D., Crielaard, W., and Hunter, C. N. (1992) Mol. Microbiol., 6, 1173-1184.
Lang, H. P., and Hunter, C. N. (1994) Biochem. J., 298, 197-205.
Moskalenko, A. A., Kuznetsova, N. Yu., and Erokhin, Yu. E. (1983) Dokl. Akad. Nauk SSSR, 269, 1248-1251.
Moskalenko, A. A. (1993) Pigment–Protein Complexes and Their Interaction in the Structures of Photosynthetic Apparatus of Bacteria and Plants: Doctoral dissertation [in Russian], IPFS RAN, Pushchino.
Moskalenko, A. A., Makhneva, Z. K., Zhuravleva, Z. A., and Erokhin, Yu. E. (2002) Dokl. Ross. Akad. Nauk, 382, 836-839.
Moskalenko, A. A., Toropygina, O. A., Zhuravleva, Z. A., and Erokhin, Yu. E. (2001) Dokl. Ross. Akad. Nauk, 381, 1-4.
Moskalenko, A. A., Britton, G., Konnor, A., Yang, A., and Toropygina, O. (1991) Biol. Membr. (Moscow), 8, 249-260.
Moskalenko, A. A., Toropygina, O. A., and Kuznetsova, N. Yu. (1995) J. Photochem. Photobiol., 27, 199-207.
Davidson, E., and Cogdell, R. J. (1981) Biochim. Biophys. Acta, 635, 295-303.
Frank, H. A., Farhoosh, R., Aldema, M. L., DeCoste, B., Christensen, R. L., Gebhard, R., and Lugtenburg, J. (1993) Photochem. Photobiol., 57, 49-55.
Frank, H. A., Chynwat, V., Hartwich, G., Meyer, M., Katheder, I., and Scheer, H. (1993) Photosynth. Res., 37, 193-203.
Bautista, J. A., Chynwat, V., Cua, A., Jansen, F. J., Lugtenburg, J., Gosztola, D., Wasilevski, M. R., and Frank, H. A. (1998) Photosynth. Res., 55, 49-65.
Jirsakova, V., and Reiss-Husson, F. (1994) FEBS Lett., 353, 151-154.
Shkuropatov, A. Ya., Khatypov, R. A., Shkuropatova, V. A., Zvereva, M. G., Owens, T. O., and Shuvalov, V. A. (1999) FEBS Lett., 450, 163-167.
Miller, J. F., Hinchigeri, S. B., Parkes-Loach, P. S., Callahan, P. M., Sprinkle, J. P., Riccobono, J. R., and Loach, P. A. (1987) Biochemistry, 26, 5056-5062.
Chang, M. C., Meyer, L., and Loach, P. A. (1990) Photochem. Photobiol., 52, 873-881.
Heller, B. A., and Loach, P. A. (1990) Photochem. Photobiol., 51, 621-627.
Fiedor, L., Leupold, D., Teuchner, K., Voigt, B., Hunter, C. N., Scherz, A., and Scheer, H. (2001) Biochemistry, 40, 3737-3747.
Razjivin, A. A., Moskalenko, A. A., and Novodereszhkin, V. I. (2000) IUBMB LIFE, 50, 115-117.
Sanadman, G. (1994) J. Biochem., 223, 7-24.
Goodwin, T. W., and Osman, H. G. (1953) Biochem. J., 53, 541-546.
Cohen-Basire, G., and Stanier, R. Y. (1958) Nature, 181, 250-252.
Britton, G., Singh, R. K., Malhotra, H. C., Goodwin, T. W., and Ben-Aziz, A. (1977) Phytochemistry, 16, 1561-1566.
Schmidt, K. (1971) Arch. Microbiol., 77, 231-238.
Schmidt, K. (1978) in Photosynthetic Bacteria (Clayton, R. K., and Sistrom, W. R., eds.) Plenum Press, New York, pp. 729-750.
Moskalenko, A. A. (1974) The Study on the Pigment–Lipoprotein Complexes from Chromatium minutissimum: Candidate's dissertation [in Russian], Minsk.
Moskalenko, A. A., and Erokhin, Yu. E. (1981) The Structural Role of Carotenoids in the Organization of the Pigment–Protein Complexes of Purple Photosynthetic Bacteria [in Russian], Pushchino, ONTI, pp. 1-20.
Moskalenko, A. A., and Karapetyan, N. V. (1996) Z. Naturforsch., 51c, 763-771.
Cogdell, R. J., and Frank, H. A. (1987) Biochim. Biophys. Acta, 895, 63-79.
Zurdo, J., Fernandez-Cabrera, C., and Ramirez, J. M. (1993) Biochem. J., 290, 531-537.
Chadwick, B. W., Zhang, C., Cogdell, R. J., and Frank, H. A. (1987) Biochim. Biophys. Acta, 893, 444-451.
Frank, H. A., and Cogdell, R. J. (1996) Photochem. Photobiol., 63, 257-264.
Cogdell, R. J., Fyfe, P. K., Howard, T. D., Fraser, N., Isaaks, N. W., Freer, A. A., McKludkey, K., and Prince, S. M. (1999) in The Photochemistry of Carotenoids (Frank, H. A., Young, A. J., Britton, G., and Cogdell, R. J., eds.) Kluwer Academic Publisher, Dordrecht, pp. 71-80.
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Toropygina, O.A., Makhneva, Z.K. & Moskalenko, A.A. Reconstitution of Carotenoids into the Light-Harvesting Complex B800–850 of Chromatium minutissimum . Biochemistry (Moscow) 68, 901–911 (2003). https://doi.org/10.1023/A:1025755116593
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DOI: https://doi.org/10.1023/A:1025755116593