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Tissue reactions of subcutaneously implanted mixture of ε-caprolactone-lactide copolymer and tricalcium phosphate. An electron microscopic evaluation in sheep

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Abstract

Biodegradable polymers, mainly derivates of α-hydroxy acids, are widely used today in oral- and maxillofacial surgery, orthopedics, and other fields of surgery. These biomaterials are well tolerated by living tissue and fracture fixation devices made of polylactic or polyglycolic acid are clinically widely used today. Still, there are some problems in application of biodegradable polymers. Abacterial inflammatory reactions have been noticed after the clinical introduction of these devices. Both swelling and pain at the site of implantation have also been reported. The etiology of this inflammatory reaction is still unknown, despite the numerous studies. Therefore, the aim of the present study was to further characterize this inflammatory reaction in detail, by electronmicroscopy. We prepared a mixture of ε-caprolactone–lactide copolymer and tricalcium phosphate and placed it in the dermis in 12 sheep. Follow-up times were 9, 14, 24, and 52 weeks. We found that the mixture caused a mild inflammatory reaction. There were no signs of cell damage. Fibroblasts, macrophages, and eosinophils were found adjacent to the copolymer. The mixture is easy to handle and can be moulded into different shapes in room temperature. The results encourage us to continue our studies to develop a filling material for bone defects.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Helsinki University Central Hospital, Finland

    Marja Ekholm, Jarkko Muhonen, Christian Lindqvist & Riitta Suuronen

  2. Department of Oral Pathology, Institute of Dentistry, University of Helsinki, Helsinki, Finland

    Marja Ekholm & Jarkko Hietanen

  3. Oral Pathology Unit/Laboratory Diagnostics, Helsinki University Central Hospital, Finland

    Jarkko Hietanen

  4. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland

    Riitta-Mari Tulamo

  5. Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Helsinki, Finland

    Christian Lindqvist

  6. Institute of Biomaterials, Tampere University of Technology, Tampere, Finland

    Minna Kellomäki

Authors
  1. Marja Ekholm
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  2. Jarkko Hietanen
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  3. Riitta-Mari Tulamo
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  4. Jarkko Muhonen
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  5. Christian Lindqvist
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  6. Minna Kellomäki
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  7. Riitta Suuronen
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Ekholm, M., Hietanen, J., Tulamo, RM. et al. Tissue reactions of subcutaneously implanted mixture of ε-caprolactone-lactide copolymer and tricalcium phosphate. An electron microscopic evaluation in sheep. Journal of Materials Science: Materials in Medicine 14, 913–918 (2003). https://doi.org/10.1023/A:1025642912270

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