Digestive Diseases and Sciences

, Volume 48, Issue 9, pp 1762–1766 | Cite as

Colonic Hydrogen Elimination and Methane Production in Infants with and Without Infantile Colic Syndrome

  • Amir Belson
  • Avinash K. Shetty
  • Peter D. Yorgin
  • Yoram Bujanover
  • Yochanan Peled
  • Mor H. Dar
  • Shimon Reif


Our objective was to investigate the relationship between demographic factors, nutrition, stool gas production, and the existence of infantile colic (IC) syndrome. Hydrogen and methane production from stool specimens of infants with and without infantile colic was quantified at two separate time points, the age at presentation of colic (<12 weeks) and at 6 months of age. The relationship between demographic variables and IC was also studied. A total of 59 infants with ages ranging from 2 to 12 weeks were enrolled in the study. Of these, 30 infants developed symptoms of colic. No correlation was found between IC and birth weight, gestational age, sex, type of feeding, mean time of feeding, stool frequency, and consistency. There was also no correlation between IC and the parents' age or education or the infant's number of siblings. Analysis of the stool samples revealed that methane was produced at concentrations >2 ppm by 15.3% of the infants at age <3 months and by 46.4% of infants at age >6 months. The mean methane concentrations produced by stool increased with age (0.95 ± 0.58 ppm at 3 months of age vs 1.29 ± 0.65 ppm at 6 months of age. There was no difference in stool hydrogen concentration between infants with and without IC. In contrast, the mean methane level at 3 and 6 months of age was higher in infants without IC than with IC, but reached statistical significance only at 6 months of age (0.97 ± 0.68 vs 0.93 ± 0.46) (NS) at 3 months of age, and 1.56 ± 0.55 vs 0.93 ± 0.62 (P<0.05) at 6 months of age respectively. Furthermore, infants that produced higher methane levels at 3 and 6 months of age had significantly (p<0.05) less colic in the first months of life. In conclusion, methane production may play a role in the alleviation of IC. Future studies are needed to confirm our findings.

infantile colic hydrogen methane 


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  1. 1.
    Brazelton TM: Crying in infancy. Pediatrics 29:579–588, 1962Google Scholar
  2. 2.
    Wessel MA, Cobb CJ, Jackson EB, Harris GS, Detweller A: Paroxysmal fussing in infancy, sometimes called “colic.” Pediatrics 14:421–434, 1954Google Scholar
  3. 3.
    Treem WR: Infant colic: a pediatric gastroenterologist's perspective. Pediatr Clin North Am 41:1121–1138, 1994Google Scholar
  4. 4.
    Jorup S: Colonic hyperperistalsis in neurolabile infants: studies in so-called dyspepsia in breast-fed infants. Acta Paediatr 85(suppl):593–599, 1952Google Scholar
  5. 5.
    Miller JJ, McVeagh P, Fleet GH, Petocz P, Brand JC: Breath hydrogen excretion in infants with colic. Arch Dis Child 64:725–729, 1989Google Scholar
  6. 6.
    Moore DJ, Robb TA, Davidson GP: Breath hydrogen response to milk containing lactose in colicky and noncolicky infants. J Pediatr 113:979–984, 1988Google Scholar
  7. 7.
    Hyams JS, Geertsma MA, Etienne NL, Treem WR: Colonic hydrogen production in infants with colic. J Pediatr 115:592–594, 1989Google Scholar
  8. 8.
    Liebman WM: Infantile colic: association with lactose and milk intolerance. JAMA: 245:732–733, 1981Google Scholar
  9. 9.
    Barr RG, Clogg IJ, Woolridge JA, Tansey CM: Carbohydrate change has no effect on infant crying behavior: a randomized controlled trial. Am J Dis Child 141:391, 1087 (Abstract)Google Scholar
  10. 10.
    Miller JJ, McVeagh P, Fleet GH, Petocz P, Brand JC: Effect of yeast lactase enzyme on “colic” in infants fed human milk. J Pediatr 117:261–263, 1990Google Scholar
  11. 11.
    Levitt MD, Bond JH: Intestinal gas. In MH Sleisenger, JS Fordtran, (eds). Gastrointestinal Disease: Pathophysiology, Diagnosis and Treatment, 3rd ed., Philadelphia, WB Saunders, 1983, p 221Google Scholar
  12. 12.
    Cummings JH, Englyst HN: Fermentation in the human large intestine and the available substrates. Am J Clin Nutr 45:1243–1255, 1987Google Scholar
  13. 13.
    Stephen AM, Haddad AC, Philips SF: Passage of carbohydrate into the colon. Direct measurements in humans. Gastroenterology 85:589–595, 1983Google Scholar
  14. 14.
    Wolin MJ, Miller TL: Carbohydrate fermentation: In Human Intestinal Microflora in Health and Disease. DJ Hentges (ed). New York, Academic Press, 1983, pp 147–165Google Scholar
  15. 15.
    Strocchi A, Levitt MD: Factors affecting hydrogen production and consumption by human fecal flora: the critical role of hydrogen tension and methanogenesis. J Clin Invest 89:1304–1311, 1994Google Scholar
  16. 16.
    Strocchi A, Ellis CJ, Furne KJ, Levitt MD: Study of constancy of hydrogen-consuming flora of human colon. Dige Dis Sci 39:494–497, 1992Google Scholar
  17. 17.
    Strocchi A, Furne JK, Ellis CJ, Levitt MD: Competition for hydrogen by human fecal bacteria. Evidence for the predominance of methane producing bacteria. Gut 32:1498–1501, 1992Google Scholar
  18. 18.
    Gibson GR, Cummings JH, Macfarlane GT, Allison C, Segal I, Vorster HH, Walker ARP: Alternative pathways for hydrogen disposal during fermentation in the human colon. Gut 31:679–683, 1990Google Scholar
  19. 19.
    Strocchi A, Ellis CJ, Levitt MD: Use of metabolic inhibitors to study H2 consumption consumption by human feces: evidence for a pathway other than methanogenesis and sulfate reduction. J Clin Invest 121:320–327, 1993Google Scholar
  20. 20.
    Lajoie SF, Bank S, Miller TL, Volin MJ: Acetate production from hydrogen and [(13)C] carbon dioxide by the microflora of human feces. Appl Environ Microbiol 54:2723–2727, 1988Google Scholar
  21. 21.
    McKay LF, Eastwood MA, Brydon WG: Methane excretion in man—a study of breath, flatus and feces. Gut 26:69–74, 1985Google Scholar
  22. 22.
    Peled Y, Gilat T, Liberman E, Bujanover Y: The development of methane production in childhood and adolescence. J Pediatr Gastroenterol Nutr 4:575–579, 1985Google Scholar
  23. 23.
    Bond JH, Engel RR, Levitt MD: Factors influencing pulmonary methane excretion in man. J Exp Med 133:572–588, 1971Google Scholar
  24. 24.
    Leuyng DTY, Robertsaw AM, Tadesse K: Breath methane exretion in Hong Kong Chinese children. J Pediatr Gastoenterol Nutr 14:275–278, 1992Google Scholar
  25. 25.
    Peled Y, Bujanover Y, Gilat T: Fecal hydrogen production in vitro: the effect of oxygen and storage. Isr J Med Sci 18:885–888, 1982Google Scholar
  26. 26.
    Norusis MJ. SPSS/PC+ Version 5.0. Chicago, Illinois, SPSS Inc., 1992Google Scholar
  27. 27.
    Hanley JH, McNeil BJ: The meaning and use of the area under receiver operating characteristic (ROC) curve. Radiology 143:29–36, 1982Google Scholar
  28. 28.
    Forsyth BMC, Leventhal JM, McCarthy PL: Mother's perceptions of problems of feeding and crying behaviors: a prospective study. Am J Dis Child 90:14–21, 1992Google Scholar
  29. 29.
    Barr RG. Colic. In WA Walker, PR Durie, JR Hamilton, JA Walker-Smith, JB Watkins (Eds.) Pediatric Gastrointestinal Disease. St. Louis, Missouri, Mosby-Year Book, 1996, pp 241–250Google Scholar
  30. 30.
    McGill AR: Infantile colic. Is it a gut issue? Pediatr Clin North Am 38:1407–1423, 1991Google Scholar
  31. 31.
    Stahlberg MR, Savilahti E: Infantile colic and feeding. Arch Dis Child 61:1232–1233, 1986Google Scholar

Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  • Amir Belson
  • Avinash K. Shetty
  • Peter D. Yorgin
  • Yoram Bujanover
  • Yochanan Peled
  • Mor H. Dar
  • Shimon Reif

There are no affiliations available

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