Skip to main content
SpringerLink
Log in

Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA

Fish Physiology and Biochemistry volume 26, pages 189–195 (2002)Cite this article

Abstract

The pharmacokinetics of ivermectin in the serum of cultured sea bream, Sparus aurata, after a single intraperitoneal injection of 100 μg kg−1 body weight was studied by the use of direct competitive ELISA. Pharmacokinetic analysis of the serum concentrations versus time points obtained was performed using non-comparmental analysis and a compartmental pharmacokinetic model approach. In the latter case a two-compartment open model with a lag time gave the best fitting. The maximum peak serum concentration was 308.4 ng ml−1 at 2 h post treatment. The AUC of ivermectin was 10700 ng h ml−1 and the elimination half-life 15.37 h, indicating a rapid uptake, high bioavailability and fast elimination of the drug by sea bream.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (Canada)

References

  • Alvinerie, M., Sutra, J.F. and Galtier, P. 1993. Ivermectin in goat plasma and milk after subcutaneous injection. Vet. Res. 24: 417–421.

    PubMed  CAS  Google Scholar 

  • Baraka, O.Z., Mahmoud, B.M., Marschke, C.K., Geary, T.G., Homeida, M.M.A. and Williams, J.F. 1996. Ivermectin distribution in the plasma and tissues of patients infected with Onchocerca volvulus. Eur. J. Clin. Pharmacol. 50: 407–410.

    Article  PubMed  CAS  Google Scholar 

  • Brown, R.D. and Manno, J.E. 1978. ESTRIP, a BASIC computer program for obtaining initial polyexponential parameter estimates. J. Pharm. Sci. 67: 1687–1695.

    PubMed  CAS  Google Scholar 

  • Chiu, S. H., Sestokas, E., Taub, R., Buhs, R. P., Green, M., Sestokas, R., Vandenheuvel, W. J., Arison, B. H. and Jacob, T. A. 1986. Metabolic disposition of ivermectin in tissues of cattle, sheep, and rats. Drug Metab Dispos. 14: 590–600.

    PubMed  CAS  Google Scholar 

  • Chukwudebe, A.C., Andrew, N., Drottar, K., Swigert, J. and Wislocki, P.G. 1996 Bioaccumulation potential of 4″-epi-(methylamino)-4″-deoxyavermectin B1a benzoate (emamectin benzoate) in bluegill sunfish. J. Agric Food Chem. 44(9): 2894–2899.

    Article  CAS  Google Scholar 

  • Craven, J., Bjorn, H., Hennessy, D., Friis, C. and Nansen, P. 2001. Pharmacokinetics of moxidectin and ivermectin following intravenous injection in pigs with different body compositions. J. Vet. Pharmacol. Ther. 24: 99–104.

    Article  PubMed  CAS  Google Scholar 

  • Crooks, S.R.H., Baxter, A.G., Traynor, I.M., Elliott, C.T. and Mc-Caughey, W.J. 1998. Detection of ivermectin residues in bovine liver using an enzyme immunoassay. Analyst 123: 355–358.

    Article  CAS  Google Scholar 

  • Davies, I.M. and Rodger, G.K. 2000. A review of the use of ivermectin as a treatment for sea lice [Lepeophtheirus salmonis (Kroyer) and Caligus elongatus Nordmann] infestation in farmed Atlantic salmon (Salmo salar L.). Aquaculture Res. 31: 869–883.

    Article  Google Scholar 

  • Daurio, C.P., Cheung, E.N., Jeffcoat, A.R. and Skelly, B.J. 1992. Bioavailability of ivermectin administered orally to dogs. Vet. Res. Commun. 16: 125–130.

    Article  PubMed  CAS  Google Scholar 

  • Degroodt, J.M., de Bukanski, B. W. and Srebrnik S. 1994. Determination of ivermectin residues in meat and liver by HPLC and fluorometric detection. J. Liq. Chromatogr. 17(6): 1419–1426.

    CAS  Google Scholar 

  • Edwards, G. 1987. Pharmacokinetics of antifilarial drugs. Trop. Med. Parasitol. 38: 64–65.

    Google Scholar 

  • Edwards, G. and Breckenridge, A.M. 1988. Clinical pharmacokinetics of anthelmitic drugs. Clin. Pharmacokinet. 15: 67–93.

    PubMed  CAS  Google Scholar 

  • Fink, D.W. and Porras, A.G. 1989. Pharmacokinetics of ivermectin in animals and humans. In: Ivermectin and Abamectin. pp. 113–130. Edited by W.C. Cambell. Springer-Verlag, New York.

    Google Scholar 

  • Gibaldi, M. and Perrier, D. 1982. Multicompartment Models. In: J. Swarbrick Eds. Pharmacokinetics, Second edition, revised and expanded. New York and Basel, Marcel Dekker, Inc., pp. 45–111.

    Google Scholar 

  • Hoy, T., Horberg, T. E. and Nafstad, I. 1990. The disposition of ivermectin in Atlantic salmon (Salmo salar). Pharmacol. Toxicol. 67: 307–312.

    Article  PubMed  CAS  Google Scholar 

  • Iosifidou, E., Shearan, P. and O'Keeffe, M. 1994. Application of the matrix solid phase dispersion technique for the determination of ivermectin residues in fish muscle tissue. Analyst 119: 2227–2229.

    Article  PubMed  CAS  Google Scholar 

  • Katharios, P., Iliopoulou-Georgudaki, J., Kapata-Zoumbos, K. and Spiropoulos, S. 2001. Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata. Fish Physiol. Bioch. 25: 99–108.

    Article  CAS  Google Scholar 

  • Kennedy, D.G., Cannavan, A., Hewitt, S.A., Rice, D.A. and Blanchflower, W.J. 1993. Determination of ivermectin residues in the tissue of Atlantic salmon (Salmo salar) using HPLC with fluorescent detection. Food Add. Contam. 10(5): 579–584.

    CAS  Google Scholar 

  • Lanusse, C., Lifschitz, A., Virkel, G., Alvarez, L., Sanchez, S., Sutra, J. F., Galtier, P., and Alvinerie, M. 1997. Comparative plasma disposition kinetics of ivermectin, moxidectin and doramectin in cattle. J. Vet. Pharmacol. Ther. 20: 91–99.

    Article  PubMed  CAS  Google Scholar 

  • Lanusse, C. and Prichard, R. 1993. Relationship between pharmacological properties and clinical efficacy of ruminant anthelmitics. Vet. Parasitol. 49: 123–158.

    Article  PubMed  CAS  Google Scholar 

  • Lifschitz, A., Virkel, G., Pis, A., Imperiale, F., Sanchez, S., Alvarez, L., Kujanek, R. and Lanusse, C. 1999. Ivermectin disposition kinetics after subcutaneous and intramuscular administration of an oil-based formulation to cattle. Vet. Parasitol. 86: 203–215.

    Article  PubMed  CAS  Google Scholar 

  • Lo, P.K.A., Fink, D.W., Williams, J.B. and Blondiger, J. 1985. Pharmacokinetic studies of ivermectin: Effects of formulation. Vet. Res. Commun. 9: 251–268.

    Article  PubMed  CAS  Google Scholar 

  • Lorio, W.J. 1989. Experimental control of metacercariae of the yellow grub Clinostomum marginatum in channel catfish. J. Aquat. Anim. Health 1: 269–271.

    Article  Google Scholar 

  • Oukessou, M., Berrag, B. and Alvinerie, M. 1999. A comparative kinetic study of ivermectin and moxidectin in lactating camels (Camelus dromedaries). Vet. Parasitol. 83: 151–159.

    Article  PubMed  CAS  Google Scholar 

  • Palmer, R., Rodger, H., Drinan, E., Dwyer, C. and Smith, P.R. 1987. Preliminary trials on the efficacy of ivermectin against parasitic copepods of Atlantic salmon. Bull. Eur. Ass. Fish Pathol. 7: 47–54.

    Google Scholar 

  • Papoutsoglou, S. 1975. Study of the metazoan parasites of the fish of Saronikos Gulf. Ph. D. Thesis, University of Patras.

  • Perez, R., Cabezas, I., Garcia, M., Rubilar, L., Sutra, J.F., Galtier, P. and Alvinerie, M. 1999. Comparison of the pharmacokinetics of moxidectin (Equest) and ivermectin (Eqvalan) in horses. J. Vet. Pharmacol. Ther. 22: 174–180.

    Article  PubMed  CAS  Google Scholar 

  • Roth, M., Rae, G., McGill, A.S. and Young, K. W. 1993a. Ivermectin depuration in Atlantic salmon (Salmo salar). J. Agric. Food Chem. 41: 2434–2436.

    Article  CAS  Google Scholar 

  • Roth, M., Richards, R.H. and Somerville, C. 1993b. Current practices in the chemotherapeutic control of sea lice infestations in aquaculture. A review. J. Fish Diseases 16: 1–26.

    Article  CAS  Google Scholar 

  • Schnitzerling, H.J. and Nolan, J. 1985. Normal phase liquid chromatographic determination of nanogram quantities of ivermectin in cattle blood or plasma. J. Assoc. Off. Anal. Chem. 68(1): 36–40.

    PubMed  CAS  Google Scholar 

  • Stoffregen, D.A., Wooster, G.A., Bustos, P.R. and Badish, J.G. 1997. Multiple route and dose pharmacokinetics of enrofloxacin in juvenile Atlantic salmon. J. Vet. Pharmacol. Therap. 20: 111–123.

    Article  CAS  Google Scholar 

  • Valenzuela, A.I., Redondo, M.J., Pico, Y. and Font, G. (2000). Determination of abamectin in citrus fruits by liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr. 1–2: 57–65.

    Article  Google Scholar 

  • Valenzuela, A.I., Popa, D.S., Redondo, M.J. and Manes, J. 2001. Comparison of various chromatographic methods for the analysis of avermectin residues in citrus fruits. J. Chromatogr. A. 918: 59–65.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Unit of Pollution and Ecotoxicology, University of Patras, Rio, 26500, Greece

    P. Katharios, J. Iliopoulou-Georgudaki & V. Kantzaris

  2. Department of Pharmacy, University of Patras, Rio, 26500, Greece

    S. Antimisiaris

  3. Department of Biology, University of Crete, 71409, Heraklion, Greece

    M. Pavlidis

Authors
  1. P. Katharios
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Iliopoulou-Georgudaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Antimisiaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Kantzaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Pavlidis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Katharios, P., Iliopoulou-Georgudaki, J., Antimisiaris, S. et al. Pharmacokinetics of ivermectin in sea bream, Sparus aurata using a direct competitive ELISA. Fish Physiology and Biochemistry 26, 189–195 (2002). https://doi.org/10.1023/A:1025473904513

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025473904513

  • ELISA
  • ivermectin
  • Pharmacokinetics
  • Sparus aurata

Search

Navigation