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Abundance of plastid DNA insertions in nuclear genomes of rice and Arabidopsis

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Abstract

Pairwise comparison of whole plastid and draft nuclear genomic sequences of Arabidopsis thaliana and Oryza sativa L. ssp. indica shows that rice nuclear genomic sequences contain homologs of plastid DNA covering about 94 kb (83%) of plastid genome and including one or more full-length intact (without mutations resulting in premature stop codons) homologues of 26 known protein-coding (KPC) plastid genes. By contrast, only about 20 kb (16%) of chloroplast DNA, including a single intact plastid-derived KPC gene, is presented in the nucleus of A. thaliana. Sixteen rice plastid genes have at least one nuclear copy without any mutation or with only synonymous substitutions. Nuclear copies for other ten plastid genes contain both synonymous and non-synonymous substitutions. Multiple ESTs for 25 out of 26 KPC genes were also found, as well as putative promoters for some of them. The study of substitutions pattern shows that some of nuclear homologues of plastid genes may be functional and/or are under the pressure of the positive natural selection. The similar comparative analysis performed on rice chromosome 1 revealed 27 contigs containing plastid-derived sequences, totalling about 84 kb and covering two thirds of chloroplast DNA, with the intact nuclear copies of 26 different KPC genes. One of these contigs, AP003280, includes almost 57 kb (45%) of chloroplast genome with the intact copies of 22 KPC genes. At the same time, we observed that relative locations of homologues in plastid DNA and the nuclear genome are significantly different.

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Authors and Affiliations

  1. Royal Holloway, University of London, Egham, Surrey, TW20 0EX, UK

    Ilham A. Shahmuradov

  2. Institute of Botany, Azerbaijan National Academy of Sciences, 370073, Baku, Azerbaijan

    Yagut Yu. Akbarova & Jalal A. Aliyev

  3. Softberry Inc., 116 Radio Circle, Suite 400, Mount Kisco, NY, 10549, USA

    Victor V. Solovyev

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  1. Ilham A. Shahmuradov
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  2. Yagut Yu. Akbarova
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  4. Jalal A. Aliyev
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Correspondence to Ilham A. Shahmuradov.

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Shahmuradov, I.A., Akbarova, Y.Y., Solovyev, V.V. et al. Abundance of plastid DNA insertions in nuclear genomes of rice and Arabidopsis . Plant Mol Biol 52, 923–934 (2003). https://doi.org/10.1023/A:1025472709537

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