Abstract
The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 μmol ml−1, showed a maximum capacity of 9.1 mg Mab ml−1 and a dynamic capacity of 3.9 mg Mab ml−1. A 95% yield of electrophoretically pure anti-GM-CSF was obtained in a single step.
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Camperi, S., Iannucci, N., Albanesi, G. et al. Monoclonal antibody purification by affinity chromatography with ligands derived from the screening of peptide combinatory libraries. Biotechnology Letters 25, 1545–1548 (2003). https://doi.org/10.1023/A:1025464918453
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DOI: https://doi.org/10.1023/A:1025464918453