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Effects of dimethoate and beta-naphthoflavone on selected biomarkers of Poecilia reticulata

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Abstract

The main objective of the present study was to investigate if a battery of enzymatic biomarkers was suitable for use as effect criteria in acute toxicity tests with Poecilia reticulata. To attain this objective, the in vivo effects of dimethoate and beta-naphthoflavone on acetylcholinesterase (AChE), cytochrome P4501A-dependent monooxygenase activity of 7-ethoxyresorufin O-deethylase (EROD), glutathione S-transferases (GST), lactate dehydrogenase (LDH) and Na+-K+-ATPase activities of P. reticulata were studied. After 96 h of exposure to sublethal concentrations (0.063 mg l−1 to 1 mg l−1) of the pesticide, an inhibition of the enzymes AChE and GST, as well as an induction of LDH was observed. The compound beta-naphthoflavone significantly induced both EROD and GST. The remaining enzymes analysed were not significantly altered by the exposure to beta-naphthoflavone (0.82 mg l−1 to 1.7 mg l−1). These results suggest that in vivo toxicity tests based on the biomarkers used in this study are sensitive and present advantages to conventional acute tests based on mortality, since they were able to detect sublethal effects in a short-period of time (96 h) indicating target and/or detoxification mechanisms.

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Authors and Affiliations

  1. Instituto de Ci^encias Biomédicas de Abel Salazar, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia, Universidade do Porto, Lg, Prof. Abel Salazar, 2, 4099-003, Porto, Portugal

    M.F. Frasco & L. Guilhermino

  2. Centro Interdisciplinar de Investigaçó Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 177, 4050-123, Porto, Portugal

    M.F. Frasco & L. Guilhermino

  3. Instituto de Ciências Biomédicas de Abel, Salazar

    L. Guilhermino

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  1. M.F. Frasco
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  2. L. Guilhermino
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Frasco, M., Guilhermino, L. Effects of dimethoate and beta-naphthoflavone on selected biomarkers of Poecilia reticulata . Fish Physiology and Biochemistry 26, 149–156 (2002). https://doi.org/10.1023/A:1025457831923

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