Abstract
The effect of extraneous zinc on calf intestinal alkaline phosphatase was studied for quick reversible binding and slow irreversible binding of zinc ions at various concentrations. Under the conditions of slow binding of zinc to CIP increasing Zn2+ (less than 1.0 mM, nM/nE 1.0 × 106) inhibited enzymatic activity, and further increasing Zn2+ resulted in an increase of activity. For quick reversible binding of Zn2+, the effect on CIP activity changed at lower concentrations of substrate, indicating a complex cooperativity between Zn2+ and pNPP. Both protein intrinsic emission fluorescence and ANS-bound protein fluorescence, as well as circular dichroism spectra have shown that the binding of zinc ions changed the enzyme conformation, which was the reason for the changes in enzyme activity induced by extraneous zinc.
Similar content being viewed by others
REFERENCES
Baksh, S., Spamer, C., and Oikawa, K. (1995). Biochem. Biophys. Res. Commun. 209: 310–315.
Bierkens, J., Van de Perr, W., and Maes, J. (1998). Comp. Biochem. Physiol. 120: 29–34.
Brazil, T., Ybarra, J., and Horowitz, P. M. (1998). J. Biol. Chem. 273: 3257–3263.
Bublitz, R., Hoppe, H., and Cumme, G. A. (2001). J. Mass Spectrom. 36: 960–972.
Cavotorta, P., Giovanelli, S., and Bobba, A. (1994). Biophys. J. 66: 1174–1179.
Chaoui, A., Mazhoudi, S., and Ghorbal, M. H. (1997). Plant Sci. 127: 139–147.
Chen, Y. H., Yang, J. T., and Martiner, H. M. (1972). Biochemistry 11: 4120–4131.
Coleman, J. E. (1992). Annu. Rev. Biophys. Biomol. Struct. 21: 441–453.
Fernley, H. H. (1971). In: Boyer, P. D. (ed.), The Enzymes (Vol. 4), Academic Press, New York, pp. 417–447.
Fosset, M., Chapellet-Tordo, D., and Lazdunski, M. (1974). Biochemistry 13: 1783–1787.
Khanna, N. C., Tokuda, M., and Waisman, D. M. (1986). J. Biol. Chem. 261: 8883–8887.
Mccomb, R. B., Bower, G. N., and Posen, S. (1979). Alkaline Phosphatase, Plenum Press, New York.
Murphy, J. E., and Kantrowitz, E. R. (1994). Mol. Microbiol. 12: 351–357.
Rein, T. W., and Wilson, I. B. (1971). In: Boyer, P. D. (ed.), The Enzymes (Vol. 4), Academic Press, New York, pp. 374–415.
Schmalz, G., Arenholtbindslev, D., and Hiller, K. A. (1997). Eur. J. Oral Sci. 105: 86–91.
Semisotnov, G. V., Rodionova, N. A., and Razgulyaev, O. I. (1991). Biopolymers 31: 119–128.
Subramaniam, V., Bergenhem, N. C., and Gafni, A. (1995). Biochemistry 34: 1133–1136.
Vallee, B. L., and Auld, D. S. (1990). Biochemistry 29: 5647–5659.
Wu, H. B., and Tsou, C. L. (1993). Biochem. J. 296: 435–441.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yan, S., Liu, Y., Tian, X. et al. Effect of Extraneous Zinc on Calf Intestinal Alkaline Phosphatase. J Protein Chem 22, 371–375 (2003). https://doi.org/10.1023/A:1025394224669
Published:
Issue Date:
DOI: https://doi.org/10.1023/A:1025394224669