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Inter- and Intraspecies Conjugal Transfer of Different Plasmids in Bacilli

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Abstract

Conjugal transfer of plasmid pUB110 between different strains of bacilli was studied. The plasmid transfer was possible not only between various strains of B. subtilis, but also when many other species of bacilli served as recipients. Conjugation of a donor strain B. subtilis 19 (p19 pUB110) was accompanied by a transfer of plasmid p19 along with plasmid pUB110 to the B. subtilis recipient strains lacking a large plasmid p19. If, like the donor cells, the recipient B. subtilis strain carried plasmid p19, the frequency of conjugation decreased. The small plasmid pBC16 was also capable of conjugative transfer. However, if this plasmid carried the mob gene with an inverted region, the frequency of its transmission dramatically decreased. If the donor strain contained another small plasmid, pV, which also carried the mob gene, the efficiency of transmission was partially restored.

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References

  1. Andrup, L., Damgaard, J., and Wasserman, K., Mobilization of Small Plasmids in Bacillus thuringiensis subs. israelensis Is Accompanied by Specific Aggregation, J. Bacteriol., 1993, vol. 175, no. 20, pp. 6530-6536.

    Google Scholar 

  2. Andrup, L., Conjugation in Gram-Positive Bacteria and Kinetics of Plasmid Transfer, APMIS, 1998, vol. 106, suppl. 84, pp. 47-55.

    Google Scholar 

  3. Koehler, T.M. and Thorn, C., Bacillus subtilis (natto) Plasmid pLS20 Mediates Interspecies Plasmid Transfer, J. Bacteriol., 1987, vol. 169, no. 11, pp. 5271-5278.

    Google Scholar 

  4. Lotareva, O.V., Nezametdinova, V.Z., Fedorina, E.A., et al., Conjugal Mobilization Implemented at a High Frequency by a Bacillus subtilis Environmental Isolate Carrying a Large Plasmid, Genetika (Moscow), 2001, vol. 37, no. 12, pp. 1598-1603.

    Google Scholar 

  5. Lotareva, O.V., Nezametdinova, V.Z., Fedorina, E.A., and Prozorov, A.A., Behavior of Various Plasmids in Soil Strain Bacillus subtilis 19 Capable of Conjugation, Mikrobiologiya, 2002, vol. 71, no. 4, pp. 567-569.

    Google Scholar 

  6. Andrup, L., Jorgensen, O., Wilcks, A., et al., Mobilization of “Nonmobilizable” Plasmids by the Aggregation-Mediated Conjugational System of Bacillus thuringiensis, Plasmid, 1996, vol. 36, no. 1, pp. 75-85.

    Google Scholar 

  7. Clark, A.J. and Warren, G., Conjugal Transmission of Plasmids, Annu. Rev. Genet., 1979, vol. 13, pp. 99-125.

    Google Scholar 

  8. Willets, N. and Skurray, R., The Conjugation System of F-like Plasmids, Annu. Rev. Genet., 1980, vol. 14, pp. 41-76.

    Google Scholar 

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Lotareva, O.V., Poluektova, E.U., Fedorina, E.A. et al. Inter- and Intraspecies Conjugal Transfer of Different Plasmids in Bacilli. Russian Journal of Genetics 39, 960–963 (2003). https://doi.org/10.1023/A:1025347326916

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  • DOI: https://doi.org/10.1023/A:1025347326916

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