Abstract
Artemisinin, an anti-malarial drug isolated from the annual wormwood Artemisia annua L., has a marked activity against chloroquine-resistant and chloroquine-sensitive strains of Plasmodium falciparum, and is useful in treatment of cerebral malaria. Shoot cultures of Artemisia annua L. were established on Murashige and Skoog basal medium which contained (per litre) 30 g sucrose, 0.5 mg 6-benzyladenine and 0.05 mg naphthaleneacetic acid. Using an optimized combination of sucrose (30 g/l), nitrate (45 mM), inorganic phosphate (200 mg/l), gibberellic acid (7 mg/l) and the ratio of NH4 +-N to NO3 −-N of 1:3, artemisinin production reached 26.7 mg/l after 30 days. This procedure provides a potential alternative for production of artemisinin from in vitro tissue cultures.
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Akalezi, C.O., Liu, S., Li, Q.S., Yu, J.T. & Zhong, J.J. 1999 Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochemistry 34, 639-642.
Avery, M.A., Chong, W.K.M. & Jennings-White, C. 1992 Stereoselective total synthesis of (+)-artemisinin, the antimalarial constituent of Artemisia annua L. Journal of the American Chemical Society 114, 974-979.
Duke, S.O. & Paul, R.N. 1993 Development and structure of the glandular trichomes of Artemisia annua L. International Journal of Plant Science 154, 107-118.
Geng, S., Ma, M., Ye, H.C., Liu, B.Y., Li, G.F. & Cong, K. 2001 Effects of ipt gene expression on the physiological and chemical characteristics of Artemisia annua L. Plant Science 160, 691-698.
He, X.C., Zeng, M.Y., Li, G.F. & Liang, Z. 1983 Callus induction and regeration of plantlets from Artemisia annua and changes of Qinghaosu contents. Acta Botanica Sinica 25, 87-90.
Klayman, D.L. 1985 Qinghaosu (artemisinin): an antimalarial drug from China. Science 228, 1049-1055.
Liersch, R., Soicke, H., Stehr, C. & Tullner, H.U. 1986 Formation of artemisinin during one vegetation period. Planta Medica 52, 387-390.
Liu, S. & Zhong, J.J. 1998 Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium. Process Biochemistry 33, 69-74.
Liu, C.Z., Wang, Y.C., Guo, C., Ouyang, F., Ye, H.C. & Li, G.F. 1998 Production of artemisinin by shoot cultures of Artemisia annua L. in a modified inner-loop mist bioreactor. Plant Science 135, 211-217.
Liu, C.Z., Wang, Y.C., Ouyang, F., Ye, H.C. & Li, G.F. 1997 Production of artemisinin by hairy root cultures of Artemisia annua L. Biotechnology Letters 19, 927-930.
Luo, X.D. & Shen, C.C. 1987 The chemistry, pharmacology and clinical applications of Qinghaosu (artemisinin) and its derivatives. Medicinal Research Reviews 7, 29-52.
Murashige, T. & Skoog, F. 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.
Nair, M.S.R., Acton, N., Klayman, D.L., Kendric, K., Basile, D.V. & Mante, S. 1986 Production of artemisinin in tissue culture of Artemisia annua. Journal of Natural Products 49, 504-507.
Paniego, N.B. & Giulietti, A.M. 1994 Artemisia annua L.: dedifferentiated and differentiated cultures. Plant Cell, Tissue and Organ Culture 36, 163-168.
Paniego, N.B. & Giulietti, A.M. 1996 Artemisinin production by Artemisia annua L.-transformed organ cultures. Enzyme and Microbial Technology 18, 526-530.
Qin, M.B., Li, G.Z., Ye, H.C. & Li, G.F. 1994 Induction of hairy root from Artemisia annua with Agrobacterium Rhizogenes and its culture in vitro. Acta Botany Sinica 36, 165-170.
Ravindranathan, T., Anil-Kumar, M., Menon, R. & Hiremath, S.V. 1990 Stereo-selective synthesis of artemisinin. Tetrahedron Letters 31, 755-758.
Shen, X.K., Yan, K.D. & Luo, Z.Y. 1983 Determination of artemisinin content by ultraviolet meter. Chinese Journal of Pharmaceutical Analysis 3, 24-26.
Souret, F.F., Weather, P.J. & Wobbe, K.K. 2002 The mevalonateindependent pathway is expressed in transformed roots of Artemisia annua and regulation by light and culture age. In Vitro Cellular and Developmental Biology-Plant 38, 581-588.
Tawfiq, N.K., Anderson, L.A., Roberts, M.F., Phillipson, J.D., Bray, D.H. & Warhurst, D.C. 1989 Antiplasmodial activity of Artemisia annua plant cell cultures. Plant Cell Reports 8, 425-428.
Wang, J.W. & Tan, R.X. 2002 Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium. Biotechnology Letters 24, 1153-1156.
Wang, Y.C., Zhang, H.X., Zhao, B. & Yuan, X.F. 2001 Improved growth of Artemisia annua L. hairy roots and artemisinin production under red light conditions. Biotechnology Letters 23, 1971-1973.
Weathers, P.J., Hemmavanh, D.D., Walcerz, D.Z., Cheetham, R.D. & Smith, T.C. 1997 Interactive effects of nitrate and phosphates salts, sucrose, and inoculum culture age on growth and sesquiterpene production in Artemisia annua hairy root cultures. In Vitro Cellular and Developmental Biology-Plant 33, 306-312.
Weathers, P.J., Wylouzil, B.E., Wobbe, K.K., Kim, Y.J. & Yigit, E. 1999 The biological response of hairy roots to O-2 levels in bioreactors. In Vitro Cellular and Developmental Biology-Plant 35, 286-289.
Woerdenbag, H.J., Jos, F.J., van Uden, W., Pras, N., Malingre, T.M. & Alfermann, A.W. 1993 Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L. Plant Cell, Tissue and Organ Culture 32, 247-257.
Xu, X.X., Zhu, J., Hung, D.Z. & Zhou, W.S. 1986 Total synthesis of arteannuin and deoxyarteannuin. Tetrahedron 42, 819-828.
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Liu, CZ., Guo, C., Wang, Y. et al. Factors influencing artemisinin production from shoot cultures of Artemisia annua L.. World Journal of Microbiology and Biotechnology 19, 535–538 (2003). https://doi.org/10.1023/A:1025158416832
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DOI: https://doi.org/10.1023/A:1025158416832