Skip to main content
SpringerLink
Log in

Role of Calcium Ions in Rapid Effects of L-Thyroxine on Phosphoinositide Metabolism in Rat Liver Cells

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The role of calcium ions in the L-thyroxine-induced initiation of hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) and also the course of releasing individual fractions of inositol phosphates and diacylglycerides (DAG) were studied in liver cells during early stages of the hormone effect. L-Thyroxine stimulated a rapid hydrolysis in hepatocytes of PtdInsP2 labeled with [14C]linoleic acid and [3H]inositol mediated by phosphoinositide-specific phospholipase C. This was associated with accumulation of [14C]DAG, total inositol phosphates, [3H]inositol 1,4,5-trisphosphate (Ins1,4,5P3) and [3H]inositol 1,4-bisphosphate (Ins1,4P2). Elimination of calcium ions from the incubation medium of hepatocytes did not abolish the effect of thyroxine on the accumulation of [14C]DAG and total [3H]inositol phosphates. Preincubation of liver cells with TMB-8 increased the stimulatory effect of L-thyroxine on the accumulation of [14C]DAG. During the incubation of hepatocytes in the presence of the hormone the content of 14C-labeled fatty acids did not change. The L-thyroxineinduced accumulation of [3H]Ins1,4,5P3 and [3H]Ins1,4P2 did not depend on the presence of calcium ions in the incubation medium of the cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Abdel-Latif, A. A. (1986) Pharmacol. Rev., 38, 227-272.

    Google Scholar 

  2. Lin, H. Y., Davis, F. B., Gordinier, J. K., Martino, L. J., and Davis, P. J. (1999) Am. J. Physiol., 276, 1014-1024.

    Google Scholar 

  3. Kavok, N. S., Krasilnikova, O. A., and Babenko, N. A. (2001) BMC Cell Biol., 2, 5.

    Google Scholar 

  4. Williams, S., Mesaeli, N., and Panagia, V. (1995) Ann. N. Y. Acad. Sci., 752, 187-191.

    Google Scholar 

  5. Hashizume, K., Yamauchi, K., Kobayashi, M., and Miyamoto, T. (1985) Endocrinol. Jpn, 32, 481-487.

    Google Scholar 

  6. Hummerich, H., and Soboll, S. (1989) Biochem. J., 258, 363-367.

    Google Scholar 

  7. Melin, P. M., Sundler, R., and Jergil, B. (1986) FEBS Lett., 198, 85-88.

    Google Scholar 

  8. Hofman, S. L., and Majerus, P. W. (1982) J. Biol. Chem., 275, 14359-14363.

    Google Scholar 

  9. Kanaeva, I. A., Karyagina, A. V., Alenicheva, T. V., Burmantova, G. A., Alimov, G. A., Archakov, A. I., and Zenkovich, G. D. (1975) Tsitologiya, 17, 545-551.

    Google Scholar 

  10. Bligh, E. G., and Dyer, W. J. (1959) Can. J. Biochem. Physiol., 37, 911-917.

    Google Scholar 

  11. Andrews, W. V., and Conn, P. M. (1987) Meth. Enzymol., 141, 156-168.

    Google Scholar 

  12. De Boland, A. R., Morelli, S., and Boland, R. (1994) J. Biol. Chem., 269, 8675-8679.

    Google Scholar 

  13. Jones, M. J., and Murray, A. W. (1995) J. Biol. Chem., 270, 5007-5013.

    Google Scholar 

  14. Rhee, S. G., and Bae, Y. S. (1997) J. Biol. Chem., 272, 15045-15048.

    Google Scholar 

  15. Bertagnolo, V., Mazzoni, M., Ricci, D., Carini, C., Neri, L. M., Previati, M., and Capitani, S. (1995) Cell Signal., 7, 669-678.

    Google Scholar 

  16. Grobler, J. A., and Hurley, J. H. (1998) Biochemistry, 37, 5020-5028.

    Google Scholar 

  17. Pawelczyk, T., and Matecki, A. (1998) Eur. J. Biochem., 257, 169-177.

    Google Scholar 

  18. Segal, J. (1990) Endocrinology, 126, 2693-2702.

    Google Scholar 

  19. Lin, H. Y., Shih, A., Davis, F. B., and Davis, P. J. (1999) Biochem. J., 338, 427-432.

    Google Scholar 

  20. Nunez, J. (1989) in Hormones and Their Actions (Cooke, B. A., King, R. J. B., and van der Molen, H. J., eds.) Elsevier Science Publishers, Amsterdam, pp. 61-80.

    Google Scholar 

  21. Berridge, M. J. (1986) Biol. Chem. Hoppe-Seyler, 367, 447-456.

    Google Scholar 

  22. Igwe, O. J., and Filla, M. B. (1995) Neuroscience, 69, 1239-1251.

    Google Scholar 

  23. Nishizuka, Ya. (1987) in Prospects in Biochemical Studies [Russian translation], Mir, Moscow, pp. 100-106.

    Google Scholar 

  24. Cobbold, P. H., Cuthbertson, K. S. R., and Woods, N. M. (1987) in Inositol Lipids Cell Signal, Banbury Meeting, 1987, Cold Spring Harbor, N. Y., pp. 119-124.

  25. Kavok, N. S., Krasil'nikova, O. A., Sidorkina, O. M., and Babenko, N. A. (2000) Biochemistry (Moscow), 65, 1331-1336.

    Google Scholar 

  26. Besterman, J. M., Pollenz, R. S., Booker, E. L., Jr., and Cuatrecasas, P. (1986) Proc. Natl. Acad. Sci. USA, 83, 9378-9382.

    Google Scholar 

  27. Sakane, F., Yamada, K., Kanoh, H., Yokoyama, C., and Tanabe, T. (1990) Nature, 344, 345-348.

    Google Scholar 

  28. Bell, R. L., Kennerly, D. A., Stanford, N., and Majerus, P. W. (1979) Proc. Natl. Acad. Sci. USA, 76, 3238-3241.

    Google Scholar 

  29. Tsubokawa, H., Oguro, K., Robinson, H. P., Masuzawa, T., and Kawai, N. (1996) J. Physiol., 497, 67-78.

    Google Scholar 

  30. Laxminarayan, K. M., Chan, B. K., Tetaz, T., Bird, P. I., and Mitchell, C. A. (1994) J. Biol. Chem., 269, 17305-17310.

    Google Scholar 

  31. Pitcher, J. A., Freedman, N. J., and Lefkowitz, R. J. (1998) Ann. Rev. Biochem., 67, 653-692.

    Google Scholar 

  32. Auethavekiat, V., Abrams, C. S., and Majerus, P. W. (1997) J. Biol. Chem., 272, 1786-1790.

    Google Scholar 

  33. Krasil'nikova, O. A., and Babenko, N. A. (1996) Biochemistry (Moscow), 61, 1008-1014.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biology, Kharkov National University, pl. Svobody 4, Kharkov, 61077, Ukraine

    O. A. Krasilnikova, N. S. Kavok & N. A. Babenko

Authors
  1. O. A. Krasilnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. S. Kavok
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. A. Babenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krasilnikova, O.A., Kavok, N.S. & Babenko, N.A. Role of Calcium Ions in Rapid Effects of L-Thyroxine on Phosphoinositide Metabolism in Rat Liver Cells. Biochemistry (Moscow) 68, 776–782 (2003). https://doi.org/10.1023/A:1025087017636

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025087017636

Search

Navigation