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A New Anionic Micellar Mobile Phase System for the Normal-Phase Thin-Layer Chromatography of Amino Compounds: Simultaneous Separation of Aminophenol Isomers

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Abstract

The chromatography of some amino compounds was performed on high-performance thin-layer plates (silica-gel 60 F254, catalog no. 1.05554, Merck, Germany) using aqueous and alcoholic (MeOH) and aqueous–organic solutions of cationic, anionic, and nonionic surfactants. The results obtained with 0.01 M aqueous sodium dodecyl sulfate (SDS) have been compared with distilled water (i.e., zero SDS) as the eluent. The effects of the surfactant concentration, the nature of the alcohol, and the presence of inorganic salts (NaCl, LiCl, CaCl2, and CoCl2) in the mobile phase were examined in order to understand the mobility pattern of amino compounds. Among the added salts, CaCl2 was found to be the most effective for facilitating an analytically important separation of coexisting ortho-, meta-, and para-aminophenols. The TLC system consisting of a precoated high-performance silica layer as the stationary phase and 0.01 M methanolic SDS plus 0.1 M CaCl2 in a 3 : 7 ratio as the mobile phase was identified as the most suitable system for the separation of o-, m-, and p-APHS from their mixtures. The influence of various impurities, such as amines, phenols, and inorganic cations, on the mobility and the separation of coexisting aminophenol isomers has also been examined. The lower limits of detection of aminophenols were determined on HPTLC plates using I2 vapors as the detection reagent.

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REFERENCES

  1. Armstrong, D.W. and Terrill, R.Q., Anal. Chem., 1979, vol. 51, p. 2160.

    Google Scholar 

  2. Armstrong, D.W. and McNeely, M., Anal. Lett., 1979, vol. 12, p. 1285.

    Google Scholar 

  3. Borgerding, M.F., Williams, R.L., Jr., Hinze, W.L., and Quina, F.H., J. Liq. Chromatogr., 1989, vol. 12, p. 1367.

    Google Scholar 

  4. Khaledi, M.G., J. Chromatogr., 1997, vol. 191, p. 780.

    Google Scholar 

  5. Shtykov, S.N., Sumina, E.G., Smushkina, E.V., and Tyurina, N.V., J. Planar Chromatogr., 1999, vol. 12, p. 129.

    Google Scholar 

  6. Landy, J.S. and Dorsey, J.G., Anal. Chim. Acta, 1985, vol. 178, p. 179.

    Google Scholar 

  7. Dorsey, J.G., DeEchegaray, M.T., and Landy, J.S., Anal. Chem., 1983, vol. 55, p. 924.

    Google Scholar 

  8. Khaledi, M.G., Strasters, J.K., Rodgers, A.H., and Breyer, E.D., Anal. Chem., 1990, vol. 62, p. 130.

    Google Scholar 

  9. Mohammad, A., Iraqi, E., and Khan, I.A., J. Surfactants Deterg., 1999, vol. 2, p. 23.

    Google Scholar 

  10. Mohammad, A., Charaf, J.P.S., Iraqi, E., and Agrawal, V., J. Planar Chromatogr., 2000, vol. 13, p. 12.

    Google Scholar 

  11. Geerdink, R.B.,J. Chromatogr., 1988, vol. 445, p. 273.

    Google Scholar 

  12. Kuhn, A.O. and Lederer, M., J. Chromatogr., 1988, vol. 445, p. 165.

    Google Scholar 

  13. Ajmal, M.A., Mohammad, A., and Anwar, S., J. Microchem., 1990, vol. 42, p. 206.

    Google Scholar 

  14. Iskander, M.L., Meiden, H.A.A., and Nashad, S., J. Microchem., 1987, vol. 36, p. 368.

    Google Scholar 

  15. Khulbe, K.C. and Mann, R.S., Fresenius Z. Anal. Chem., 1988, vol. 330, p. 642.

    Google Scholar 

  16. Mohammad, A. and Iraqi, E., J. Surfactants Deterg., 1999, vol. 2, p. 85.

    Google Scholar 

  17. Shalaby, A.R., Food Chem., 1999, vol. 65, p. 117.

    Google Scholar 

  18. Sherma, J., Anal. Chem., 2000, vol. 72, p. 9R.

    Google Scholar 

  19. Parihar, D.B., Sharma, S.P., and Verma, K.K., J. Chromatogr., 1967, vol. 31, p. 120.

    Google Scholar 

  20. Dwivedy, A.K., Parihar, D.B., Sharma, S.P., and Verma, K.K., J. Chromatogr., 1967, vol. 29, p. 120.

    Google Scholar 

  21. Pielesz, A., Swierczek, S., Wlochomicz, A., and Baranousfcaj, I., J. Planar Chromatogr., 1999, vol. 12, p. 215.

    Google Scholar 

  22. Naquib, K., Ayesh, A.M., and Snalaby, R., J. Agric. Food Chem., 1995, vol. 43, p. 134.

    Google Scholar 

  23. Yasuda, K., J. Chromatogr., 1972, vol. 72, p. 413.

    Google Scholar 

  24. Yasuda, K., J. Chromatogr., 1971, vol. 60, p. 144.

    Google Scholar 

  25. Ajmal, M., Mohammad, A., and Anwar, S., J. Planar Chromatogr., 1990, vol. 3, p. 336.

    Google Scholar 

  26. Mohammad, A., Ajmal, M., and Anwar, S., J. Planar Chromatogr., 1991, vol. 4, p. 319.

    Google Scholar 

  27. Mohammad, A., Ajmal, M., and Anwar, S., Indian J. Chem. Tech., 2000, vol. 7, p. 17.

    Google Scholar 

  28. Mitehell, S.C. and Waring, R.H., J. Chromatogr., 1986, vol. 358, p. 288.

    Google Scholar 

  29. Phillon, R.S., Singii, J., Gautam, V.K., and Chhabra, B.R., J. Chromatogr., 1988, vol. 435, p. 256.

    Google Scholar 

  30. Weiser, H.B., Milligan, W.O., and Bates, J.B., J. Phys. Chem., 1942, vol. 46, p. 99.

    Google Scholar 

  31. Bund, O., Fischer, W., and Hauck, H.E., J. Planar Chromatogr., 1995, vol. 8, p. 360.

    Google Scholar 

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Authors and Affiliations

  1. Analytical Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, 202002, India

    A. Mohammad & Y. H. Sirwal

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  1. A. Mohammad
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  2. Y. H. Sirwal
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Mohammad, A., Sirwal, Y.H. A New Anionic Micellar Mobile Phase System for the Normal-Phase Thin-Layer Chromatography of Amino Compounds: Simultaneous Separation of Aminophenol Isomers. Journal of Analytical Chemistry 58, 731–739 (2003). https://doi.org/10.1023/A:1025079425987

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