Biotechnology Letters

, Volume 25, Issue 15, pp 1239–1241 | Cite as

A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium

  • Yuanyuan Xu
  • Wei Du
  • Dehua LiuEmail author
  • Jing Zeng


A new enzymatic route for biodiesel production from soybean oil was developed using methyl acetate as a novel acyl acceptor. Novozym 435 (immobilized Candida antarctica lipase) gave the highest methyl ester (ME) yield of 92%. The optimum conditions of the transesterification were 30% enzyme based on oil weight; a molar ratio of methyl acetate/oil of 12:1; temperature 40 °C and reaction time 10 h. Since no glycerol was produced in the process, this method is very convenient for recycling the catalyst and by-product triacetylglycerol showed no negative effect on the fuel property.

biodiesel methyl acetate Novozym 435 transesterification 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Department of Chemical EngineeringTsinghua UniversityBeijingP.R. China

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