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In vitro micropropagation of a Cucurbita interspecific hybrid cultivar – a root stock plant

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Abstract

An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot–tips of 5-day-old explants. The excised shoot–tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l−1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1∼1.5 cm length) were rooted most effectively in 1 mg l−1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, South Korea

    S. Sarowar, H.Y. Oh, S.H. Ok & J.S. Shin

  2. Department of Horticultural Science, Sangmyung University, Chonan, 330-180, South Korea

    N.I. Hyung

  3. Biotechnology Center, Nong Woo Bio Co., Ltd., Yeoju, Kyonggi, 469-880, South Korea

    B.W. Min, C.H. Harn & S.K. Yang

Authors
  1. S. Sarowar
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  2. H.Y. Oh
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  3. N.I. Hyung
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  4. B.W. Min
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  5. C.H. Harn
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  6. S.K. Yang
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  7. S.H. Ok
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  8. J.S. Shin
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Correspondence to J.S. Shin.

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Sarowar, S., Oh, H., Hyung, N. et al. In vitro micropropagation of a Cucurbita interspecific hybrid cultivar – a root stock plant. Plant Cell, Tissue and Organ Culture 75, 179–182 (2003). https://doi.org/10.1023/A:1025043130423

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  • DOI: https://doi.org/10.1023/A:1025043130423

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