Abstract
Morphogenetic status of cactus Mammillaria gracillis Pfeiff. tissue culture was studied by light and electron microscopy. In vitro propagated shoots spontaneously developed callus. This callus regenerated normal and hyperhydric shoots without exogenous hormones. Tumour tissue induced by wild or rooty strains of Agrobacterium tumefaciens never expressed any morphogenetic potential. Light microscopy showed cellular characteristics of morphologically different tissues. Ultrastructural studies revealed changes in plastids: secondary dedifferentiation of mature chloroplasts, thylakoid swelling and disruption, phytoferritin accumulation, plastid elongation and increase in size. Changes in chlorophyll and carotenoid content were in accordance with degradation of the thylakoid system. Plastids were confirmed as very sensitive organelles to an artificial hyperhydric environment as well as to Agrobacteria-mediated cell transformation.
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References
Balen B, Miloševi? J & Krsnik-Rasol M (2002) Protein and glycoprotein patterns related to morphogenesis in Mammillaria gracillis Pfeiff. tissue culture. Food Technol. Biotech. 40: 275–280
Balen B, Krsnik-Rasol M & Simeon-Rudolf V (2003) Isoenzymes of peroxidase and esterase related to morphogenesis in Mammillaria gracillis Pfeiff. tissue culture. J. Plant Physiol. (in press) Bhau BS (1999) Regeneration of Coryphantha elephantides (Lem.) Lem. (Cactaceae) from root explants. Sci. Horticult. 81: 337–344
Birchem R, Sommer HE & Brown CL (1981) Comparison of plastids of Pinus pallustris Mill. and Pinus elliottii Engelm. in callus tissue culture. Pflanzenphysiol 102: 101–107
Caredda S, Devaux P, Sangwan RS & Clement C (1999) Differen-tial development of plastids during microspore embryogenesis in barley. Protoplasma 208: 248–256
Caredda S, Doncoeur C, Devaux P, Sangwan RS & Clement C (2000) Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.). Sex Plant Reprod. 13: 95–104
Crèvecoeur M, Kevers C, Greppin H & Gaspar T (1987) A comparative biochemical and cytological characterization of normal and habituated sugarbeet calli. Biol. Plant 29: 1–6
Crèvecoeur M, Hagège D, Catesson AM, Greppin H & Gaspar T (1992) Ultrastructural characteristics of cells from normal and habituated sugar beet calli. Plant Physiol. Biochem. 30: 87–95
Duncan DB (1955) Multiple range and multiple F-tests. Biometrics 11: 1–42
Elias-Rocha MA, Santos-Diaz MD & Arredondo-Gomez A (1998) Propagation of Mammillaria candida (Cactaceae) by tissue culture techniques. Haseltonia 6: 96–101
Freeman TP & Duysen ME (1975) The effect of imposed water stress on the development and ultrastructure of wheat chloro-plasts. Protoplasma 83: 131–145
Gaspar T (1995) The concept of cancer in in vitro plant cultures and the implication of habituation to hormones and hyperhydrici-ty. Plant Tiss. Cult. Biotech. 1: 126–136
Hagège D, Werck-Reichhart D, Schmitt P & Gaspar T (1992) Deficiency in tetrapyrrole-containing compounds in a non-organogenic habituated sugarbeet cell line. Plant Physiol. Biochem. 30: 649–654
Kirk JTO & Tilney-Bassett RAE (1978) The Plastids: Their Chemistry, Structure, Growth and Inheritance. Elsevier / North-Holland Biomedical Press, Amsterdam
Krsnik-Rasol M & Balen B (2001) Electrophoretic protein patterns and peroxidase activity related to morphogenesis in Mammillaria gracillis tissue culture. Acta Bot. Croatica 60: 219–226
Llamoca-Zarate RM, Studart-Guimaraes C, Landsmann J & Cam-pos FAP (1999a) Establishment of callus and cell suspension cultures of Opuntia ficus-indica. Plant Cell Tiss. Org. Cult. 58: 155–157
Llamoca-Zarate RM, Aguiar LF, Landsmann J & Campos FAP (1999b) Whole plant regeneration of Opuntia ficus-indica Mill. (Cactaceae). J. Appl. Bot. 73: 83–85
Le Dily FJ, Billard JP, Gaspar T & Huault C (1993) Disturbed nitrogen metabolism associated with the hyperhydric status of fully habituated callus of sugarbeet. Physiol. Plant 88: 129–134
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth. Enzym. 148: 350–382
Liners F, Gaspar T & Van Cutsem P (1994) Acetyl-and methyl-esterification of pectins of friable and compact sugar-beet calli: consequences for intercellular adhesion. Planta 192: 545–556
Lorkovi? Z, Muraja-Fras J, Krsnik-Rasol M & Wrischer M (1993) Ultrastructural and biochemical changes in potato tuber cells related to tumourigenesis. Plant Physiol. Biochem. 31: 633–638
Ljubešić N (1976) Phytoferritin in plastids of blackberry leaves. Acta Bot. Croatica 35: 51–55
Malda G, Backhaus RA & Martin C (1999) Alterations in growth and crassulacean acid matabolism (CAM) activity of in vitro cultured cactus. Plant Cell Tiss. Org. Cult. 58: 1–9
Mangolin CA, Ottoboni LMM & Machado MFPS (1999) Two-dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins. Electrophoresis 20: 626–629
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15: 473–479
Sjolund RD & Weier TE (1971) An ultrastructural study of chloro-plast structure and dedifferentiation in tissue cultures of Strep-tanthus tortuosus (Cruciferae). Am. J. Bot. 58: 172–181
Srivastava LM, Vesk M & Singh AP (1971) Effect of chloram-phenicol on membrane transformation in plastids. Can. J. Bot. 49: 587–593
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Poljuha, D., Balen, B., Bauer, A. et al. Morphology and ultrastructure of Mammillaria gracillis (Cactaceae) in in vitro culture. Plant Cell, Tissue and Organ Culture 75, 117–123 (2003). https://doi.org/10.1023/A:1025030115905
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DOI: https://doi.org/10.1023/A:1025030115905