Abstract
The use of poly(methylmethacrylate) PMMA cement by Charnley in the 1960s revolutionized orthopaedic medicine. Since this time, however, little has changed. The development of bioactive composites, such as HAPEX™ (a composite of 40% vol hydroxyapatite (HA) in a polyethylene matrix) have potential in orthopaedic applications. The composite has been shown to allow direct bone bonding in vivo, and in vitro studies have shown preferential attachment to HA exposed on the composite surface. In vitro study has also shown that altering the topography HAPEX™ can enhance osteoblast response. This study uses microscopical investigation of osteoblast cytoskeleton, and biochemical measurement of proliferation (by thymidine incorporation) and phenotype (by alkaline phosphatase activity) to compare primary human osteoblast (HOB) activity on HAPEX™ and PMMA cement. The study shows large increases in HOB response to the new generation material compared to PMMA, the current implant standard.
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References
P. S. Walker, S. F. Mai, A. G. Coob, G. Bentley and J. Hua, JBJS 74B (1995) 705.
J. Charnley, JBJS 42B (1960) 28.
Y. K. Liu, J. B. Park, G. O. Njus and D. Stienstra, J. Biomed. Mater. Res. 21 (1987) 247.
T. Kindt-Larsen, D. B. Smith and J. S. Jensen, J. Appl. Biomater. 6 (1995) 75.
T. P. Harrigan, J. A. Kareth, D. O. O'Connor, D. W. Burke and W. H. Harris, J. Orthop. Res. 10 (1992) 134.
M. A. R. Freeman, G. W. Bradley and P. A. Revell, JBJS 64B (1982) 489.
J. S. Wang, H. Franzen, E. Jonsson and L. Lidgren, Acta. Orthop. Scand. 64 (1993) 143.
W. Bonfield, M. D. Grynpas, A. E. Tully, J. Bowman and J. Abram, Biomaterials 2 (1981) 185.
P. K. Stephenson, M. A. Freeman, P. A. Revell, J. Germain, M. Tuke and C. J. Pirie, J. Arthroplasty 6 (1991) 51.
H. Ohgushi, Y. Dohi, T. Katuda, S. Tamai, S. Tabata and Y. Suwa, J. Biomed. Mater. Res. 32 (1996) 333.
T. Gruen, G. M. Mcneice and H. C. Amstutz, Clin. Orthop. 141 (1979) 17.
A. S. Posner and F. Betts, Acc. Chem. Res., 8 (1975) 273.
R. N. Downes, S. Vardy, K. E. Tanner and W. Bonfield, Bioceramics 4 (1991) 239.
J. Huang, M. Wang, K. E. Tanner, W. Bonfield and L. Di Silvio, J. Mater. Sci. Mater. Med. 8 (1997) 775.
A. S. G. Curtis and C. D. W. Wilkinson, Trends Biotechnol. 19 (2001) 97.
A. M. Rajnicek and C. D. Mccraig, J. Cell. Sci. 110 (1997) 2915.
M. J. Dalby, S. J. Yarwood, M. O. Riehle, H. J. H. Johnstone, S. Affrossman and A. S. G. Curtis, Exp. Cell. Res. 276 (2002) 1.
M. J. Dalby, M. Riehle, H. J. H. Johnstone, S. Affrossman and A. S. G. Curtis, Biomaterials 23 (2002) 2945.
B. A. Dalton, M. D. M. Evans, G. A. Mcfarland and J. G. Steele, J. Biomed. Mater. Res. 45 (1999) 384.
C. Gray, A. Boyde and S. J. Jones, Bone 18 (1996) 115.
M. J. Dalby, M. V. Kayser, W. Bonfield and L. Di Silvio, Biomaterials 23 (2002) 681.
M. J. Dalby, L. Di Silvio, N. Gurav, B. Annaz, M. V. Kayser and W. Bonfield, Tissue Eng. 8 (2002) 467.
M. J. Dalby, L. Di Silvio, E. J. Harper and W. Bonfield, Biomaterials 23 (2002) 569.
M. J. Dalby, L. Di Silvio, E. J. Harper and W. Bonfield, Biomaterials 22 (2001) 1739.
M. J. Dalby, L. Di Silvio, E. J. Harper and W. Bonfield, Biomaterials 23 (2002) 569.
M. J. Dalby, L. Di Silvio, E. J. Harper and W. Bonfield, J. Mater. Sci. Mater. Med. 10 (1999) 793.
M. J. Dalby, L. Di Silvio, E. J. Harper and W. Bonfield, J. Mater. Sci. Mater. Med. 13 (2002) 311.
L. Di Silvio and N. GuravHuman Cell Culture (Kluwer Academic Publishers, London, 2001) 221.
B. Kasemo and J. Lausmaa, Environ. Health Prospects 102 (1994) 878.
L. Di Silvio, M. J. Dalby and W. Bonfield, Biomaterials 23 (2002) 101.
K. Burridge and M. Chzanowska-Wodnick, Annu. Rev. Cell Dev. Biol. 12 (1996) 463.
R. Sinha, F. Morris, A. Suken, S. Shah and R. Tuan, Clin. Orthop. 305 (1994) 258.
R. L. Juliano and S. Haskill, J. Cell. Biol. 120 (1993) 577.
P. J. Marie, Calcif. Tissue Int. 56 (1995) supplement.
E. A. Cowles, M. E. Derome, G. Pastizzo, L. L. Brailey and G. A. Gronowicz, Calcif. Tissue Int. 62 (1998) 74.
S. Y. AliBone Biology and Skeletal Disorders (Carfax, Abingdon, UK, 1992) 19.
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Dalby, M.J., Bonfield, W. & Di Silvio, L. Enhanced HAPEX topography: Comparison of osteoblast response to established cement. Journal of Materials Science: Materials in Medicine 14, 693–697 (2003). https://doi.org/10.1023/A:1024959632082
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DOI: https://doi.org/10.1023/A:1024959632082