Skip to main content
SpringerLink
Log in

Molecular cloning and characterization of a novel mannose-binding lectin gene from Amorphophallus konjac

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

A new lectin gene was cloned from Amorphophallus konjac. The full-length cDNA of Amorphophallus konjac agglutinin (aka) was 736 bp and contained a 474 bp open reading frame encoding a 158 amino acid protein. Homology analysis revealed that the lectin from this Araceae species belonged to the superfamily of monocot mannose-binding proteins. Molecular modeling of AKA indicated that the three-dimensional structure of AKA strongly resembles that of the snowdrop lectin. Southern blot analysis of the genomic DNA revealed that aka belonged to a low-copy gene family. Northern blot analysis demonstrated that aka expression was tissue-specific with the strongest expression being found in root.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Van Damme EJM, Peumans WJ, Barre B & Rouge P (1998) Plant Lectins: Critical Rev. Plant Sci. 17: 1–112

    Google Scholar 

  2. Van Damme EJM, Hanae K, Fulvio P, Irwin J, Goldstein, Ben P, Fumio Y, Benny D & Willy JP (1991) Eur. J. Biochem. 202: 23–30

    Google Scholar 

  3. Van Damme EJM, Smeets K, Torrekens S, Van Leuven F & Peumans W.J (1994) Eur. J. Biochem. 221: 769–777

    Google Scholar 

  4. Van Damme EJM, Goossens K, Smeets K, Van Leuven F, Verhaert P & Peumans WJ (1995) Plant Physiol. 107: 1147–1158

    Google Scholar 

  5. Van Damme EJM, Brike F, Winter HC, Van Leuven F, Goldstein IJ & Peumans WJ (1996) Eur. J. Biochem. 236: 419–427

    Google Scholar 

  6. Smeets K, Van Damme EJM, Verhaert P, Barre A, Rouge P, Van Leuven F & Peumans WJ (1997) Plant Mol. Biol. 33: 223–234

    Google Scholar 

  7. Smeets K, Van Damme EJM, Van Leuven F & Peumans WJ (1997) Plant Mol. Biol. 35: 531–535

    Google Scholar 

  8. Yagi F, Hidaka M, Minami Y & Tadera K (1996) Plant Cell Physiol. 37: 1007–12

    Google Scholar 

  9. Peumans WJ, Barre A, Bras J, Rouge P, Proost P & Van Damme EJ (2002) Plant Physiol. 129: 1054–1065

    Google Scholar 

  10. Ooi LS, Ng TB, Geng Y & Ooi VE (2000) Biochem. Cell Biol. 78: 463–368

    Google Scholar 

  11. Hirai M, Nakamura K, Imai T & Sato T (1993) Jpn. J. Genet. 68: 229–236

    Google Scholar 

  12. Powell KS, Gatehouse, AMR, Hilder VA & Gatehouse JA (1995) Entomol. Exp. Appl. 75: 51–59

    Google Scholar 

  13. Huang DF, Pan YH, Zhang SX, Cao JP, Yang XM, Zhang J & Yin WZ (1997) Sci. Agri. Sin. 30: 94–98

    Google Scholar 

  14. Pan YH, Zhang SX, Cao JP & Huang DF (1998) Progress Nat. Sci. 8: 502–505

    Google Scholar 

  15. Van Damme EJM, Anthony KA & Peumans WJ (1987) FEBS Lett. 215: 140–144

    Google Scholar 

  16. Mao X, Gao LF, Li CX & Li RZ (1999) J. Shangxi Agric. Univ. 19: 122–124

    Google Scholar 

  17. Li RZ, Gao WJ, Ji DP & Li CX (2000) Acta Gossypii Sin. 12: 54–56

    Google Scholar 

  18. Van Damme EJM, Barre A, Rouge P, Van Leuven F, Balzarini J & Peumans WJ (1996) Plant Mol. Biol. 31: 657–72

    Google Scholar 

  19. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA & Struhl K (1995) Short protocols in molecular biology. John Wiley & Sons, Inc. p2.3.1

  20. Van Heijine G (1986) Nucl. Acids Res. 14: 4683–4690

    Google Scholar 

  21. Altschul SF, Madden TL, Schaffer AA, Zhang J, Miller W & Lipman DJ (1997) Nucl. Acids Res. 25: 3389–3402

    Google Scholar 

  22. Wang X, Bauw G, Van Damme, EJ, Peumans WJ, Chen ZL, Van Montagu M, Angenon G & Dillen W (2001) Plant J. 25: 651–661

    Google Scholar 

  23. Guex N & Peitsch MC (1997) Electrophoresis 18: 2714–2723

    Google Scholar 

  24. Barre A, Bourne Y, Van Damme EJM, Peumans WJ & Rougé P (2001) Biochimie 83: 645–651

    Google Scholar 

  25. Hester G, Kaku H, Goldstein IJ & Wright CS (1995) Nat Struct. Biol. 2: 472–479

    Google Scholar 

  26. Van Damme EJM, Goldstein IJ, Vercammen G & Peumans WJ (1992) Plant Physiol. 86: 245–252

    Google Scholar 

  27. Hilder VA, Powell KS, Gatehouse AMR & Gatehouse JA (1995) Transgenic Res. 4: 18–25

    Google Scholar 

  28. Peumans WJ & Van Damme EJ (1995) Plant Physiol. 109: 347–52

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Plant Biotechnology Center, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200030, China

    Jiong Fei, Jiong Fei, Zhihua Liao, Yourong Chai, Yongzhen Pang, Jianhong Yao, Xiaofen Sun, Xiaofen Sun, Kexuan Tang & Kexuan Tang

Authors
  1. Jiong Fei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhihua Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yourong Chai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yongzhen Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianhong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaofen Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kexuan Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fei, J., Liao, Z., Chai, Y. et al. Molecular cloning and characterization of a novel mannose-binding lectin gene from Amorphophallus konjac . Mol Biol Rep 30, 177–183 (2003). https://doi.org/10.1023/A:1024956525396

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024956525396

Search

Navigation