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Role of Different Isoforms of Nitric Oxide Synthase in Development of Tumor Mutants in Drosophila melanogaster

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Abstract

We studied the role of nitric oxide synthase during tumor growth in oncovirus-induced tumor mutants of Drosophila melanogaster. The lines with different capacity for malignancy differed reliably in the level of enzymatic activity. It was shown using specific inhibitors of neuronal and inducible isoforms that the neuronal isoform was not involved in tumor formation, while the inducible one appears to play an important role in tumor growth inhibition. This isoform was identified with the help of immunoblotting and monoclonal antibodies against inducible nitric oxide synthase.

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Authors and Affiliations

  1. Al-Farabi Kazakh National University, prosp. Al-Farabi 71, Almaty, 480078, Kazakhstan

    L. B. Dzhansugurova, B. O. Bekmanov & R. I. Bersimbaev

  2. Institute of General Genetics and Cytology, Ministry of Education and Science, prosp. Al-Farabi 75, Almaty, 480060, Kazakhstan

    L. B. Dzhansugurova, B. O. Bekmanov & R. I. Bersimbaev

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  1. L. B. Dzhansugurova
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  2. B. O. Bekmanov
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  3. R. I. Bersimbaev
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Dzhansugurova, L.B., Bekmanov, B.O. & Bersimbaev, R.I. Role of Different Isoforms of Nitric Oxide Synthase in Development of Tumor Mutants in Drosophila melanogaster . Russian Journal of Developmental Biology 34, 254–261 (2003). https://doi.org/10.1023/A:1024952701377

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  • DOI: https://doi.org/10.1023/A:1024952701377

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