Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates

Abstract

The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.

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Correspondence to Andrew W. Lloyd.

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van der Valk, A.M., Howbrook, D.N., O'Shaughnessy, M.C. et al. Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates. Biotechnology Letters 25, 1325–1328 (2003). https://doi.org/10.1023/A:1024951322955

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  • alkaline phosphatase
  • biotin
  • fluorescein
  • polypropylene
  • streptavidin