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Physical separation of chlorophyll-protein complexes

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Abstract

This minireview focuses on the physical separation of chlorophyll-protein complexes and particles from the two photosystems by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) or differential/sucrose density-gradient centrifugation during the decade of 1960s.

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References

  • Anderson JM (2002) Changing concepts about the distribution of Photosystems I and II between grana-appressed and stromaexposed thylakoid membranes. Photosynth Res 73: 157–164

    Article  PubMed  CAS  Google Scholar 

  • Bassham JA (2003) Mapping the carbon reduction cycle: a personal retrospective. Photosynth Res 76: 35–52 (this issue)

    Article  PubMed  Google Scholar 

  • Bengis C and Nelson N (1975) Purification and properties of Photosystem I reaction center from chloroplasts. J Biol Chem 250: 2783–2788

    PubMed  CAS  Google Scholar 

  • Benson AA (2002) Following the path of carbon in photosynthesis: a personal story. Photosynth Res 73: 29–49

    Article  PubMed  CAS  Google Scholar 

  • Berthold DA, Babcock GT and Yocum CF (1981) A highly resolved oxygen-evolving Photosystem II preparation from spinach thylakoid membranes. FEBS Lett 134: 231–234

    Article  CAS  Google Scholar 

  • Boardman NK and Anderson JM (1964) Isolation from spinach chloroplasts of particles containing different proportions of chlorophyll a and b: their possible role in light reactions of photosynthesis. Nature 203: 166–167

    Article  CAS  Google Scholar 

  • Briantais J-M (1967) Retablissement du lien entre deux structures chloroplastiques isolees par action du Triton X-100. Biochim Biophys Acta 143: 650–653

    Article  PubMed  CAS  Google Scholar 

  • Burke JJ, Ditto CL and Arntzen CJ (1978) Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys 187: 252–264

    Article  PubMed  CAS  Google Scholar 

  • Friends of K. Shibata (1984) In memory of Kazuo Shibata. In: Sybesma C (ed) Advances in Photosynthesis Research, Vol I, pp XXIII–XXIV. Martinus Nijhoff/Dr Junk Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Govindjee (1986) The discovery of chlorophyll-protein complex by Emil L. Smith during 1931-1941. Photosynth Res 16: 291–292

    Google Scholar 

  • Haworth P, Watson JL and Arntzen CJ (1983) The detection, isolation and characterization of a light-harvesting complex, which is specifically associated with Photosystem I. Biochim Biophys Acta 724: 151–158

    Article  CAS  Google Scholar 

  • Jagendorf AT (2002) Photophosphorylation and the chemiosmotic perspective. Photosynth Res 73: 233–241

    Article  PubMed  CAS  Google Scholar 

  • Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauss N (2001) Three-dimensional structure of cyanobacterial Photosystem I at 2.5 Å resolution. Nature 411: 909–917

    Article  PubMed  CAS  Google Scholar 

  • Katoh S (2003) Early research on the role of plastocyanin in photosynthesis. Photosynth Res 76: 255–261 (this issue)

    Article  PubMed  CAS  Google Scholar 

  • Nanba O and Satoh K (1987) Isolation of a Photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci USA 84: 109–112

    Article  PubMed  CAS  Google Scholar 

  • Nelson N and Ben-Shem A (2002) Photosystem I reaction center: past and future. Photosynth Res 73: 193–206

    Article  PubMed  CAS  Google Scholar 

  • Ogawa T (1991) A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC 6803. Proc Natl Acad Sci USA 88: 4275–4279

    Article  PubMed  CAS  Google Scholar 

  • Ogawa T, Obata F and Shibata K (1966) Two pigment proteins in spinach chloroplasts. Biochim Biophys Acta 112: 223–234

    PubMed  CAS  Google Scholar 

  • Ogawa T, Kanai R and Shibata K (1967) Distribution of carotenoids in the two photochemical systems of higher plants and algae. In: Shibata K, Takamiya A, Jagendorf AT and Fuller RC (eds) Comparative Biochemistry and Biophysics of Photosynthesis, pp 22–35. Tokyo University Press, Tokyo

    Google Scholar 

  • Ogawa T, Vernon LP and Mollenhauer HH (1969) Properties and structure of fractions prepared from Anabaena variabilis by the action Triton X-100. Biochim Biophys Acta 172: 216–229

    Article  PubMed  CAS  Google Scholar 

  • Ogawa T, Ishikawa H, Shimada K and Shibata K (1978) Synergistic action of red and blue light and action spectra for malate formation in guard cells of Vicia faba L. Planta 142: 61–65

    Article  CAS  Google Scholar 

  • Satoh K (2003) The identification of the Photosystem II reaction center: a personal story. Photosynth Res 76: 233–240 (this issue)

    Article  PubMed  CAS  Google Scholar 

  • Shibata M, Ohkawa H, Kaneko T, Fukuzawa H, Tabata S, Kaplan A and Ogawa T (2001) Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria: genes involved and their phylogenetic relationship with homologous genes in other organisms. Proc Natl Acad Sci USA 98: 11789–11794

    Article  PubMed  CAS  Google Scholar 

  • Smith EL (1938) Solution of chlorophyll-protein compounds (phyllochlorin) extracted from spinach. Science 88: 170–171

    CAS  Google Scholar 

  • Tamiya H (1966) Synchronous culture of algae. Ann Rev Plant Physiol 17: 1–26

    Article  Google Scholar 

  • Thornber JP, Gregory RPF, Smith CA and Bailey JL (1967) Studies on the nature of the chloroplast lamella. I. Preparation and some properties of two chlorophyll-protein complexes. Biochemistry 6: 391–396

    Article  PubMed  CAS  Google Scholar 

  • Vernon LP (2003) Photosynthesis and the Charles F. Kettering Research Laboratory. Photosynth Res 76: 379–388 (this issue)

    Article  PubMed  CAS  Google Scholar 

  • Vernon LP, Shaw ER and Ke B (1966) A photochemically active particle derived from chloroplasts by the action of the detergent Triton X-100. J Biol Chem 241: 4101–4109

    PubMed  CAS  Google Scholar 

  • Vernon LP, Yamamoto HY and Ogawa T (1969) Partially purified photosynthetic reaction centers from plant tissues. Proc Natl Acad Sci USA 63: 911–914

    Article  PubMed  CAS  Google Scholar 

  • Vernon LP, Shaw ER, Ogawa T and Raveed D (1971) Structure of Photosystem I and Photosystem II of plant chloroplasts. Photochem Photobiol 14: 343–357

    CAS  Google Scholar 

  • Wessels JSC (1962) Separation of the two photochemical systems of photosynthesis by digitonin fragmentation of spinach chloroplast. Biochim Biophys Acta 65: 561–564

    Article  PubMed  CAS  Google Scholar 

  • Zouni A, Witt HT, Kern J, Fromme P, Krauss N, Saenger W and Orth P (2001) Crystal structure of Photosystem II from Synechococcus elongatus at 3.8 Å resolution. Nature 409: 739–743

    Article  PubMed  CAS  Google Scholar 

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  1. Bioscience Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan (

    Teruo Ogawa

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  1. Teruo Ogawa
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Ogawa, T. Physical separation of chlorophyll-protein complexes. Photosynthesis Research 76, 227–232 (2003). https://doi.org/10.1023/A:1024943228292

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