Abstract
Replication initiation proceeds in a random fashion in early development of Xenopus laevis. The replication origins become fixed only at later stages of development after the mid-blastula transition. Specification of replication origins occurs at the same time with the specification of the DNA attachment to the nuclear matrix. Replication origins of many species coincide or are located in the vicinity of sites of DNA attachment to the nuclear matrix. The present work was dedicated to development of an experimental system where DNA loops were specifically attached to an artificial matrix and a study of an effect of this attachment on specificity of DNA replication initiation in extracts of Xenopus laevis oocytes. We have found that DNA attachment to the artificial matrix increases the efficacy of DNA replication as compared to the control, but does not affect the replication specificity. It is likely that the transition from non-specific to specific replication is determined by a combination of several factors, and specificity of DNA attachment to a matrix alone is not sufficient for specification of a replication origin.
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Amati, B., Pick, L., Laroche, T., and Gasser, S. M., Nuclear Scaffold Attachment Stimulates, But Is Not Essential for ars Activity in Saccharomyces cerevisiae: Analysis of the Drosophila ftz sar, EMBO J., 1990, vol. 9, pp. 4007-4016.
Blow, J. J. and Sleeman, A. M., Replication of Purified DNA in Xenopus Egg Extract Is Dependent on Nuclear Assembly, J. Cell Sci., 1990, vol. 95, pp. 383-391.
Buongiorno-Nardelli, M., Micheli, G., Carri, M. T., and Marilley, M., A Relationship between Replicon Size and Supercoiled Loop Domains in the Eukaryotic Genome, Nature, 1982, vol. 298, pp. 100-102.
Hyrien, O., Maric, C., and Mechali, M., Transition in Speci-fication of Embryonic Metazoan DNA Replication Origins, Science, 1995, vol. 270, pp. 994-997.
Lohka, M. J. and Matsui, Y., Formation in vitro of Sperm Pronuclei and Mitotic Chromosomes Induced by Amphibian Ooplasmic Components, Science, 1983, vol. 220, pp. 719-721.
Mechali, M. and Kearsey, S., Lack of Specific Sequence Requirement for DNA Replication in Xenopus Eggs Compared with High Sequence Specificity in Yeast, Cell, 1984, vol. 38, pp. 55-64.
Murray, A. W., Cell Cycle Extracts, Methods in Cell Biology, 1991, vol. 36, pp. 581-605.
Newport, J., Nuclear Reconstitution in vitro: Stages of Assembly around Protein-Free DNA, Cell, 1987, vol. 48, pp. 205-217.
Razin, S. V., Kekelidze, M. G., Lukanidin, E. M., et al., Replication Origins Are Attached to the Nuclear Skeleton, Nucl. Acids Res., 1986, vol. 14, pp. 8189-8207.
van der Velden, H. M., van Willigen, G., Wetzels, R. H., and Wanka, F., Attachment of Origins of Replication to the Nuclear Matrix and the Chromosomal Scaffold, FEBS Lett., 1984, vol. 171, pp. 13-16.
Vassetzky, Y. S., Nuclear Matrix and Replication of Plasmid DNA in Higher Eukaryotes, Russ. J. Dev. Biol., 1997, vol. 28, pp. 445-450.
Vassetzky, Y., Hair, A., and Mechali, M., Rearrangement of Chromatin Domains during Development in Xenopus, Genes Dev., 2000a, vol. 14, pp. 1541-1552.
Vassetzky, Y., Lemaitre, J. M., and Mechali, M., Specification of Chromatin Domains and Regulation of Replication and Transcription during Development, Crit. Rev. Eukaryot. Gene Expr., 2000b, vol. 10, pp. 31-38.
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Vassetzky, Y.S. Attachment of DNA Loops to an Artificial Matrix Does Not Affect the Replication Origin Specificity in Early Development of Xenopus laevis . Russian Journal of Developmental Biology 34, 213–217 (2003). https://doi.org/10.1023/A:1024940331419
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DOI: https://doi.org/10.1023/A:1024940331419