Abstract
The study of cell reaction to micro and nanotopography is dependent on the method of manufacture available. Several methods of manufacture have been developed: polymer demixing, embossing and photolithography. Surfaces obtained with these different techniques, having micro and/or nanodomains, have been studied toward the same type of cells, i.e. human endothelial cells (HGTFN) and mouse fibroblasts (3T3). Polymer demixing of polystyrene (PS) and poly(4-bromostyrene) (PBrS) producing nanometrically islands of 18, 45 and 100 nm height, polycarbonate (PC) and polycaprolactone (PCL) grooved with grooves 450 nm wide and 190 high, the natural polysaccharide hyaluronic acid (Hyal) and its sulfated derivative (HyalS) photoimmobilized on silanized glass as grooves 250 nm high and 100, 50, 25 or 10 μm wide have been obtained. The morphology and polarization of the cells has been studied by optical microscopy and scanning electron microscopy. Cells respond in different way to the topography of the materials, but the surface chemistry is dominant in inducing different cell behavior.
Similar content being viewed by others
References
O. D. Velvev and E. W. Kaler, Langmuir 15 (1999) 11.
P. Ghosh, W. M. Y. Lackowhisky and R. M. Crooks, Macromolecules 33 (2000) 590.
T. A. Desai, Med. Eng. Physics 22 (2000) 595.
A. S. G. Curtis and M. Riehle, Phys. Med. Biol. 46 (2001) R47.
R. G. Flemming, C. J. Murphy, G. S. Abrams, S. L. Goodmann and P. F. Nealey, Biomaterials 20 (1999) 573.
S. Walheim, M. Boltau, J. Mlynek, G. Krausch and U. Steiner, Macromolecules 30 (1997) 4995.
S. Affrossman, G. Henn, S. A. O'Neill, Pethrick and M. Stamm, ibid. 29 (1996) 5010.
G. Chen, Y. Ito, Y. Imanishy, A. Magnani, S. Lamponi and R. Barbucci, Bioconj. Chem. 8 (1997) 730.
M. J. Dalby, S. J. Yarwood, M. O. Riehle, J. H. Heather, S. Johnstone Affrossman and A. S. G. Curtis, Exp. Cell Res. 276 (2002) 1.
R. Barbucci, S. Lamponi, A. Magnani and D. Pasqui, J. Eng. Materials Sci. 19 (2002) 161.
R. Barbucci, A. Magnani, S. Lamponi, D. Pasqui and S. Bryan, Biomaterials 24 (2003) 915.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Barbucci, R., Pasqui, D., Wirsen, A. et al. Micro and nano-structured surfaces. Journal of Materials Science: Materials in Medicine 14, 721–725 (2003). https://doi.org/10.1023/A:1024919917969
Issue Date:
DOI: https://doi.org/10.1023/A:1024919917969