An Improved On-Line HPLC-DPPH Method for the Screening of Free Radical Scavenging Compounds in Water Extracts of Lamiaceae Plants

Abstract

An on-line HPLC-1,1-diphenyl-2-picrylhydrazyl (DPPH*) method has been improved for the detection of polar and nonpolar radical scavenging compounds from complex plant extracts. Eight water extracts were prepared from steam-distilled essential oil-extracted Lamiaceae plants (Origanum vulgare L., O. Onites L., O. Minutiflorum O. Schwartz et P. H. Davis, O. Syriacum L., Satureja cuneifolia Ten., Thymbra spicata L., Coridothymus capitatus (L.) Reichb. f., Majorana hortensis Moench). After the components within each extract had been separated by reverse phase chromatography using 10% to 100% methanol with 2% acetic acid as a mobile phase, analytes capable of scavenging a citric acid-sodium citrate buffered methanolic DPPH* solution were detected by post-column derivatization at 517 nm. The HPLC-DPPH* on-line method was applied to the qualitative and quantitative analysis of these Lamiaceae plant extracts. There was a strong correlation between the scavenging (negative) peak area and the concentration of the radical scavenging reference substances used. The radical scavenging compounds within the extracts were determined as benzoic acid and hydroxycinnamic acid derivatives, flavonoids and diterpenoids according to their retention time and UV spectral data. Rosmarinic acid and carnosic acid were identified as the dominant radical scavengers in these extracts by this method.

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REFERENCES

  1. 1.

    E. J. Park and J. M. Pezzuto, In: Encyclopedia of Pharmaceutical Technology, J. Scarbrick and J. C. Boylan eds., Marcel Dekker Incorporated, USA (2001).

    Google Scholar 

  2. 2.

    S.-Y. Wang, Y.-H. Kuo, H.-N. Chang, P.-L. Kang, H.-S. Tsay, K.-F. Lin, N.-S. Yang, and L.-F. Shyur, J. Agric. Food Chem., 50, 1859 (2002).

    Google Scholar 

  3. 3.

    F. D. Goffman and C. Mollers, J. Agric. Food Chem., 48, 1605 (2000).

    Google Scholar 

  4. 4.

    Q. Chen, H. Shi, and C.-T. Ho., JAOCS, 69, 999 (1992)

    Google Scholar 

  5. 5.

    F. Shahidi and M. Naczk, In: Food Phenolics Sources, Chemistry, Effects, Applications. Lancester, USA: Technomic Publishing Co., Inc., 1995, p. 235–277.

    Google Scholar 

  6. 6.

    S. Sang, K. Lapsley, W.-S. Jeong, P. A. Lanchance, C.-T. Ho, and R. T. Rosen, J. Agric. Food Chem., 50, 2459 (2002).

    Google Scholar 

  7. 7.

    M. Wang, J. Li, M. Rangarajan, Y. Shao, E. J. LaVoie, T.-C. Huang, and C-T. Ho, J. Agric. Food Chem. 46, 4869 (1998).

    Google Scholar 

  8. 8.

    F. Shahidi, P. K. Janitha, and P. D. Wanasundara, Crit. Rev. Food Sci., 32, 67 (1992).

    Google Scholar 

  9. 9.

    H.-S. Choi, H. S. Song, H. Ukeda, and M. Sawamura, J. Agric. Food Chem., 48, 4156 (2000).

    Google Scholar 

  10. 10.

    X. C. Weng and W. Wang, Food Chem., 71, 489 (2000).

    Google Scholar 

  11. 11.

    A. Dapkevicius, R. Venskutonis, T. A. van Beek, and J. P. H. Linssen, J. Sci. Food Agric., 77, 140 (1998).

    Google Scholar 

  12. 12.

    A. Escarpa and M. C. Gonzalez, Eur. Food Res. Technol., 212, 439 (2001).

    Google Scholar 

  13. 13.

    L. Pizzale, R. Bortolomeazzi, S. Vichi, E. Uberegger, and L. C. Conte, J. Sci. Food Agric., 82, 1645 (2002).

    Google Scholar 

  14. 14.

    K. Hostettmann, J.-L. Wolfender, and C. Terreaux, Pharm. Biol., 39, 18 (2001).

    Google Scholar 

  15. 15.

    D. Bandoniene, M. Murkovic, W. Pfannhauser, P. R. Venskutonis, and D. Gruzdiene, Eur. Food Res. Technol., 214, 143 (2002).

    Google Scholar 

  16. 16.

    A. Dapkevicius, T. A. van Beek, and H. A. G. Niederlander, J. Chrom., 912, 73 (2001).

    Google Scholar 

  17. 17.

    A. Dapkevicius, T. A. van Beek, H. A. G. Niederlander, and A. de Groot, Anal. Chem., 71, 736 (1999).

    Google Scholar 

  18. 18.

    I. I. Koleva, H. A. G. Niederlander, and T. A. van Beek, Anal. Chem., 72, 2323 (2000).

    Google Scholar 

  19. 19.

    D. Bandoniene and M. Murkovic, J. Agric. Food Chem., 50, 2482 (2002).

    Google Scholar 

  20. 20.

    D. Bandoniene and M. Murkovic, J. Biochem. Biophys. Methods, 53, 45 (2002).

    Google Scholar 

  21. 21.

    I. I. Koleva, H. A. G. Niederlander, and T.A. van Beek, Anal. Chem., 73, 3373 (2001).

    Google Scholar 

  22. 22.

    N. Bouchet, L. Barrier, and B. Fauconneau, Phytother. Res., 12, 159 (1998).

    Google Scholar 

  23. 23.

    G. Zgorka and K. Glowniak, J. Pharm. Biomed. Anal., 26, 79 (2001).

    Google Scholar 

  24. 24.

    K. Triantaphyllou, G. Blekas, and D. Boskou, Int. J. Food Sci. Nut., 52, 313 (2001).

    Google Scholar 

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Kosar, M., Dorman, H.J.D., Bachmayer, O. et al. An Improved On-Line HPLC-DPPH Method for the Screening of Free Radical Scavenging Compounds in Water Extracts of Lamiaceae Plants. Chemistry of Natural Compounds 39, 161–166 (2003). https://doi.org/10.1023/A:1024853628326

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  • Lamiaceae
  • online HPLC-DPPH*
  • antioxidants
  • radical scavenging compounds
  • 1,1-diphenyl-2-picrylhydrazyl (DPPH*)