Plant and Soil

, Volume 253, Issue 2, pp 293–299 | Cite as

Glomalin, an arbuscular-mycorrhizal fungal soil protein, responds to land-use change

  • Matthias C. RilligEmail author
  • Philip W. Ramsey
  • Sherri Morris
  • Eldor A. Paul


Glomalin is a soil proteinaceous substance produced by arbuscular mycorrhizal fungi. Most of the information available concerning this protein has been collected in relation to its role in soil aggregation. In this study, we explored the distribution of glomalin across soil horizons, decomposition of glomalin, and relationship with soil C and N in an agricultural field, a native forest, and an afforested system. Glomalin was present in A, B, and C horizons in decreasing concentrations. Land-use type significantly affected glomalin concentrations (mg cm−3), with native forest soils having the highest concentrations of the three land-use types in both A and B horizons. In terms of glomalin stocks (Mg ha−1), calculated based on corrected horizon weights, the agricultural area was significantly lower than both afforested and native forest areas. As measured after a 413 day laboratory soil incubation, glomalin was least persistent in the A horizon of the afforested area.. In agricultural soils and native soils, ca. 50% of glomalin was still remaining after this incubation, indicating that some glomalin may be in the slow or recalcitrant soil C fraction. Comparison of glomalin decomposition with CO2-C respired during incubation indicates that glomalin makes a large contribution to active soil organic C pools. Soil C and N were highly correlated with glomalin across all soils and within each land-use type, indicating that glomalin may be under similar controls as soil C. Our results show that glomalin may be useful as an indicator of land-use change effects on deciduous forest soils.

decomposition forest soil glycoprotein soil carbon storage 


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  1. Birdsey R A, Platinga A J, and Heath L S 1993 Past and prospective carbon storage in United States forests. Forest Ecol. Manage. 58, 33–40.Google Scholar
  2. Ellert B and Gregorich E G 1996 Storage of C, N, and P in cultivated and adjacent forest soils of Ontario. Soil Sci. 1619, 587–603.Google Scholar
  3. Haile-Mariam S, Cheng W, Johnson D W, Ball J T, and Paul E A. 2000 Use of carbon-13 and carbon-14 to measure the effects of carbon dioxide and nitrogen fertilization on carbon dynamics in ponderosa pine. Soil Sci. Soc. Am. J. 64: 1984–1993.Google Scholar
  4. Hungate B A, Jackson R B, Field C B and Chapin F S III 1996 Detecting changes in soil carbon in CO2 enrichment experiments. Plant and Soil 187, 135–145.Google Scholar
  5. Myneni R B, Dong J, Tucker C J, Kaufmann R K, Kauppi P E, Liski J, Zhou L, Alexeyev V and Hughes MK 2001 A large carbon sink in the woody biomass of Northern forests. Proc. Natl. Acad. Sci. USA 10, 1073–1084.Google Scholar
  6. Paul E A, Morris S J, and Böhm S 2001 The determination of soil C pool sizes and turnover rates: Biophysical fractionation and tracers. In Assessment Methods for Soil Carbon. Eds. R Lal, J M Kimble, R F Follett and B A Stewart. pp. 193–206. CRC Press LLC, Boca Raton.Google Scholar
  7. Paul E A, Morris S J, Six J, Paustian K, and Gregorich E. 2002. Determination of the controls on C and N dynamics in afforested agricultural soils. Soil Sci. Soc. Am. J. Special Issue. In review.Google Scholar
  8. Paul E A and Clark F E 1996 Soil Biology and Biochemistry. Academic Press, San Diego.Google Scholar
  9. Rillig M C, Wright S F, Shaw M R and Field C B 2002a Artificial ecosystem warming positively affects arbuscular mycorrhizae but decreases soil aggregation. Oikos 97, 52–58.Google Scholar
  10. Rillig M C, Wright S F and Eviner V 2002b The role of arbuscular mycorrhizal fungi and glomalin in soil aggregation: comparing effects of five plant species. Plant Soil 238, 325–333.Google Scholar
  11. Rillig M C, Wright S F, Nichols K A, Schmidt W F and Torn M S 2001 Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils. Plant Soil 233, 167–177.Google Scholar
  12. Rillig M C, Hernandez G Y and Newton P C D 2000 Arbuscular mycorrhizae respond to elevated atmospheric CO2 after long-term exposure: evidence from a CO2 spring in New Zealand supports the resource-balance model. Ecol. Lett. 3, 475–478.Google Scholar
  13. Rillig M C, Wright S F, Allen M F and Field C B 1999 Rise in carbon dioxide changes soil structure. Nature 400, 628.Google Scholar
  14. Six J, Callewaert P, Lenders S, Degryze S, Morris S J, Gregorich E G, Paul E A, and Paustian K 2002 Measuring and Understanding Carbon Storage in Reforested-Agricultural Soils by Physical Fractionation. Soil Sci. Soc. Am. J. 66, 1981–1987.Google Scholar
  15. Smith S E and Read D J 1997 Mycorrhizal Symbiosis. Academic Press, San Diego.Google Scholar
  16. Steinberg P D, Rillig M C 2003 Differential decomposition of arbuscular mycorrhizal fungal hyphae and glomalin. Soil Biol. Biochem. 35, 191–194.Google Scholar
  17. Wright S F and Anderson R L 2000 Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Biol. Fert. Soils 31, 249–253.Google Scholar
  18. Wright S F, Starr J L and Paltineanu I C 1999 Changes in aggregate stability and concentration of glomalin during tillage management transition. Soil Sci. Soc. Am. J. 63, 1825–1829.Google Scholar
  19. Wright S F and Upadhyaya A 1998 A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 198, 97–107.Google Scholar
  20. Wright S F and Upadhyaya A 1996 Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Sci. 161, 575–586.Google Scholar
  21. Wright S F, Franke-Snyder M, Morton J B and Upadhyaya A 1996 Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181, 193–203.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Matthias C. Rillig
    • 1
    Email author
  • Philip W. Ramsey
    • 1
  • Sherri Morris
    • 2
  • Eldor A. Paul
    • 3
  1. 1.Microbial Ecology Program, Division of Biological SciencesThe University of MontanaMissoulaUSA
  2. 2.Biology DepartmentBradley UniversityPeoriaUSA
  3. 3.Natural Resource Ecology LaboratoryColorado State UniversityFort CollinsUSA

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