Skip to main content
SpringerLink
Log in

Hyperplasia of Myoepithelial Cells Expressing Calponin During Atrophy of the Rat Parotid Gland Induced by Duct Ligation

  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Abstract

The number and location of myoepithelial cells in the rat parotid gland submitted to surgical ligation of its main excretory duct were studied through immunohistochemical labelling for calponin. These cells were labelled by the streptavidin-biotin method using anti-calponin primary antibody, and their number was determined during each step of glandular atrophy, i.e., at time zero (control) and 1, 7, 15, 21, 30 and 60 days after ligation. Morphological analysis showed a gradual decrease and fibrosis of the glandular lobules accompanied by disappearance of the acini and the occurrence of duct-like structures. The expression of calponin was observed in all specimens analysed, being restricted to myoepithelial cells. Labelling revealed the distribution of myoepithelial cells around the acini and intercalated ducts in the control group and around duct-like structures later during the course of atrophy. Quantitative analysis demonstrated significant increase in the number of myoepithelial cells up to day 7 post-ligation, followed by gradual increases which, however, were not statistically significant. These results suggest that myoepithelial cells proliferate intensely up to day 7 post-ligation, an event that coincides with a higher rate of disappearance of acinar cells. After this period, the elevated number of cells observed at the end of the previous period is maintained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Burgess KL, Dardick I (1998) Cell population changes during atrophy and regeneration of rat parotid gland. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85: 699–706.

    Google Scholar 

  • Burgess KL, Dardick I, Cummins MM, Burford-Mason AP, Bassett R, Brown DH (1996) Myoepithelial cells actively proliferate during atrophy of rat parotid gland. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 82: 674–680.

    Google Scholar 

  • Dardick I (1996) Color Atlas/Text of Salivary Gland Pathology. New York: Igaku-Shoin Medical Publishers, p. 274.

    Google Scholar 

  • Dardick I, Parks WR, Little J, Brown DL (1988) Characterization of cystoskeletal proteins in basal cells of human parotid salivary gland ducts. Virchows Arch A Pathol Anat Histopathol 412: 525–532.

    Google Scholar 

  • Denny PC, Ball WD, Redman RS (1997) Salivary glands: A paradigm for diversity of gland development. Crit Rev Oral Biol Med 8: 51–75.

    Google Scholar 

  • Emmelin N, Garrett JR, Ohlin P (1974) Secretory activity and the myoepithelial cells of salivary glands after duct ligation in cats. Arch Oral Biol 19: 275–283.

    Google Scholar 

  • Foschini MP, Eusebi V (1998) Carcinomas of the breast showing myoepithelial cell differentiation: A review of the literature. Virchows Arch 432: 303–310.

    Google Scholar 

  • Garrett JR, Emmelin N (1979) Activities of salivary mioepithelial cells. A review. Med Biol 57: 1–28.

    Google Scholar 

  • Garrett JR, Parsons PA (1973) Alkaline phosphatase and myoepithelial cells in the parotid gland of the rat. Histochem J 5: 463–471.

    Google Scholar 

  • Harrison JD, Garrett JR (1976) Histological effects of ductal ligation of salivary glands of the cat. J Pathol 118: 245–254.

    Google Scholar 

  • Loyola AM, Araújo VC, Sousa SOM (1999) Ausência de diferenciação mioepitelial em carcinomas mucoepidermóides de glândulas salivares menores. J Bras Patol 35: 190–194.

    Google Scholar 

  • Nagao T, Sugano I, Ishida Y, Tajima Y, Matsuzaki O, Konno A, Kondo Y, Nagao K (1998) Salivary gland malignant myoepithelioma: A clinicopathologic and immunohistochemical study of ten cases. Cancer 83: 1292–1299.

    Google Scholar 

  • Pagnoncelli RM, Yurgel LS (1997) Estudo histológico e macroscópico de alterações provocadas por ligadura do ducto excretor principal da glândula submandibular esquerda do rato. Rev Odonto Ciência 24: 87–107.

    Google Scholar 

  • Palmer RM (1986) The identification of myoepithelial cells in human salivary glands. A review and comparison of light microscopical methods. J Oral Pathol 15: 221–229.

    Google Scholar 

  • Prasad AR, Savera AT, Gown AM, Zarbo RJ (1999) The myoepithelial immunophenotype in 135 benign and malignant salivary gland tumors others than pleomorphic adenoma. Arch Pathol Lab Med 123: 801–806.

    Google Scholar 

  • Redman RS (1994) Myoepithelium of salivary glands. Microsc Res Tech 27: 25–45.

    Google Scholar 

  • Rice DH (1999) Noninflammatory, non-neoplastic disorders of the salivary glands. Otolaryngol Clin North Am 32: 835–842.

    Google Scholar 

  • Savera AT, Gown AM, Zarbo RJ (1997) Immunolocalization of novel smooth muscle-specific proteins in salivary gland pleomorphic adenoma: Assessment of the morphogenetic role of myoepithelium. Modern Pathol 10: 1093–1100.

    Google Scholar 

  • Savera AT, Sloman A, Huvos AG, Klimstra DS (2000) Myoepithelial carcinoma of the salivary glands:Aclinicopathologic study of 25 patients. Am J Surg Pathol 24: 761–774.

    Google Scholar 

  • Scott J, Liu P, Smith PM (1999) Morphological and functional characteristics of acinar atrophy and recovery in the duct-ligated parotid gland of the rat. J Dent Res 78: 1711–1719.

    Google Scholar 

  • Shimizu M, Tokumori K, Saitoh M, Miwa K, Yoshiuka K, Kanda S (2000) Sonographic analisys of rat submandibular glands in experimentally induced sialadenitis. Dentomaxillofac Radiol 29: 90–96.

    Google Scholar 

  • Shinohara M, Oka M, Yamada K, Hashimura K, Yuba K, Mori M (1992) Immunohistochemical and electronmicroscopic studies of obstructive lesions in submandibular glands. J Oral Pathol Med 21: 370–375.

    Google Scholar 

  • Souza LB, Taga EM, Onofre MA, Taga R (1995) Estudo estereológico da atrofia da glândula parótida do rato após ligadura do ducto excretor principal. Rev Odonto Univ São Paulo 9: 1–9.

    Google Scholar 

  • Souza AP, Trevilato PC, Zaia AA, Line SRP (1999) Análise bioquímica das metaloproteases da matriz extracelular durante atrofia experimental das glândulas salivares submandibulares em ratos. Rev Odonto Univ São Paulo 13: 135–139.

    Google Scholar 

  • Standish SM, Shafer WG (1957) Serial histologic effects of rat submaxillary and sublingual salivary gland duct and blood vessel ligation. J Dent Res 36: 866–879.

    Google Scholar 

  • Takahashi K, Hiwada K, Kokubu T (1988) Vascular smooth muscle calponin. A novel troponin T-like protein. Hypertension 11: 620–626.

    Google Scholar 

  • Takahashi S, Nakamura S, Suzuki R, Domon T, Yamamoto T, Wakita M (1999) Changing myoepithelial cell distribution during regeneration of rat parotid glands. Int J Exp Path 80: 283–290.

    Google Scholar 

  • Takahashi S, Schoch S, Walker NI (1998) Origin of acinar cell regeneration after atrophy of the rat parotid induced by duct obstruction. Int J Exp Path 79: 293–301.

    Google Scholar 

  • Tamarin A(1967) Secretory cell alterations associated with submaxillary gland duct ligation. In: Schneyers LH, Schneyers CA, eds. Secretory Mechanisms of Salivary Glands. New York: Academic Press, pp. 220–237.

    Google Scholar 

  • Tamarin A (1971) Submaxillary gland recovery from obstruction. II. Electron microscopic alterations of acinar cells. J Ultrastruct Res 34: 288–302.

    Google Scholar 

  • Walker NI, Gobé GC (1987) Cell death and cell proliferation during atrophy of the rat parotid gland induced by duct obstruction. J Pathol 153: 333–344.

    Google Scholar 

  • Zaia AA, Almeida OP, Line SRP (1997) Immunochemical analysis of laminin in duct ligated submandibular glands of rats. J Oral Pathol Med 26: 451–453.

    Google Scholar 

  • Zarbo RL, Prasad AR, Regezi JA, Gown AM, Savera AT (2000) Salivary gland basal cell and canalicular adenomas: Immunohistochemical demonstration of myoepithelial cell participation and morphogenetic considerations. Arch Pathol Lab Med 124: 401–405.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Oral Pathology, Dentistry School, Federal University of Rio Grande do Norte, 1787-CEP-59056-000, Natal, Brazil

    M.C.C. Miguel, E.S.S. Andrade, L.P. Pinto & L.B. Souza

  2. Laboratory of Histology, Department of Biological Sciences, Dentistry School of Bauru, University of São Paulo, São Paulo, Brazil

    R. Taga

Authors
  1. M.C.C. Miguel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E.S.S. Andrade
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Taga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L.P. Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L.B. Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miguel, M., Andrade, E., Taga, R. et al. Hyperplasia of Myoepithelial Cells Expressing Calponin During Atrophy of the Rat Parotid Gland Induced by Duct Ligation. Histochem J 34, 499–506 (2002). https://doi.org/10.1023/A:1024761923303

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024761923303

Keywords

Search

Navigation