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Modified synthesis of deoxyadenosine-5'-(35S)-thiomonophosphate [dAMP (35S)] with high specific activity: An important starting material for the synthesis of deoxyadenosine-5'-(&;#945;-35S)-thiotriphosphate [dATP (&;#945;-35S)]

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Abstract

Deoxyadenosine-5'-monophosphite was treated with excess of trimethylsilyl chloride. One equivalent of trimethylsilyl chloride treated phosphite in pyridine and one equivalent of elemental 35S in toluene solution (specific activity > 1000 Ci/mmol) were stirred together with gradual removal of solvents under vacuum, over a period of one hour, to give deoxyadenosine-5'-(35S)-thiomonophosphate [dAMP(35S)] in high yields.

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Authors and Affiliations

  1. ILCJP, Board of Radiation and Isotope Technology, BARC Trombay, Mumbai, 400 085, India

    H. Chander, S. Ravi & K. M. Mathew

Authors
  1. H. Chander
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  2. S. Ravi
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  3. K. M. Mathew
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Chander, H., Ravi, S. & Mathew, K.M. Modified synthesis of deoxyadenosine-5'-(35S)-thiomonophosphate [dAMP (35S)] with high specific activity: An important starting material for the synthesis of deoxyadenosine-5'-(&;#945;-35S)-thiotriphosphate [dATP (&;#945;-35S)]. Journal of Radioanalytical and Nuclear Chemistry 257, 425–426 (2003). https://doi.org/10.1023/A:1024716820462

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