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A rapid and efficient method for regeneration of plantlets from embryo explants of cumin (Cuminum cyminum)

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Abstract

A new, simple and efficient method was developed for multiple shoot regeneration of cumin from imbibed embryo cultures. This method yielded a large number of shoots within short period of time (30–50 days) without any subculturing. The effects of different media, different embryo explants and various combinations of plant growth regulators (PGRs) on callus formation and shoot regeneration in cumin were investigated. Simultaneous callus formation and shoot regeneration was obtained. The best response for multiple shoot regeneration was observed on B5 medium containing 1.0 mg l−1 BAP, 0.2 mg l−1 NAA and 0.4 mg l−1 IAA, with an average of 140 shoots per explant.

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Authors and Affiliations

  1. Department of Tissue Culture and Transformation Agricultural Biotechnology Research Institute of Iran, (ABRII), P.O. Box 31535-1897, Karaj, Iran

    Esmaeil Ebrahimie, A.A. Habashi, B. Ghareyazie & M. Mohammadie

  2. Department of Crop Production and Plant Breeding, University of Tehran, Karaj, Iran

    M. Ghannadha

Authors
  1. Esmaeil Ebrahimie
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  2. A.A. Habashi
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  3. B. Ghareyazie
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  4. M. Ghannadha
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  5. M. Mohammadie
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Correspondence to A.A. Habashi.

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Ebrahimie, E., Habashi, A., Ghareyazie, B. et al. A rapid and efficient method for regeneration of plantlets from embryo explants of cumin (Cuminum cyminum). Plant Cell, Tissue and Organ Culture 75, 19–25 (2003). https://doi.org/10.1023/A:1024676507010

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