Abstract
A group of olive varieties, commonlycultivated in the sub-coastal area of theEmilia-Romagna region, in Italy's northernolive cultivation area limit, weredescribed using both morphological andmolecular approaches. The morpho-biologicalcharacters of each accession were collectedaccording to the UPOV's descriptor list(International Union for the Protection ofNew Varieties of Plants). Biometric indexesof inflorescence, leaf, fruit, and stone,measured using the Video Image Analysis(VIA), were compared to the molecular dataobtained on the same set of cultivars usingAFLP (Amplified Fragment LengthPolymorphism) and SSR (Simple SequenceRepeat) markers. The morphological andmolecular data led to similarrepresentations of the cultivarrelationships. However, only the AFLP andSSR data were able to characterize specificolive varieties and identify erroneousdenominations and cases of synonymy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amane, M., R. Lumaret, V. Hany, N. Ouazzani, C. Debain, G. Vivier & M.F. Deguilloux, 1999. Chloroplast-DNA variation in cultivated and wild olive (Olea europaea L.). Theor Appl Genet 99: 133-139.
Angiolillo, A., M. Mencuccini & L. Baldoni, 1999. Olive (Olea europaea L.) genetic diversity assessed by Amplified Fragment Length Polymorphisms. Theor Appl Genet 98: 411-421.
Barranco, D. & L Rallo, 1985. Las variedades de olivo cultivades en Espana. Olivae 9: 16-22.
Barranco, D., A. Cimato, P. Fiorino, L. Rallo, A. Touzani, C. Castañeda, F. Serafín & I. Trujillo, 2000. World Catalogue of olive varieties. International Olive Oil Council. Madrid, Spain, 360 pp.
Barranco, D. & L. Rallo, 2000. Olive Cultivars in Spain. Hortechnology 10(1): 107-110.
Bartolini, G., G. Prevost, C. Messeri, G. Carignani & U.G. Menini, 1998. Olive Germplasm. Cultivars and World-Wide Collections. FAO, Rome.
Belaj, A., I. Trujillo, R. De la Rosa & L. Rallo, 2000. Polymorphism and discrimination capacity of randomly amplified polymorphic markers in an olive germplasm bank. J Amer Soc Hort Sci 126(1): 64-71.
Besnard, G. & A. Bervillé, 2000. Multiple origins for Mediterranean olive (Olea europaea L. ssp. europaea) based upon mitochondrial DNA polymorphisms. Life Sciences 323: 173-181. CR Acad Sci Paris.
Besnard, G., P. Baradat & A. Bervillé, 2001. Genetic relationships in the olive (Olea europaea L.) reflect multilocal selection of cultivars. Theor Appl Genet 102(2/3): 251-258.
Besnard, G., B. Khadari, P. Villemur & A. Bervillé, 2000. Cytoplasmic male sterility in the olive (Olea europaea L.). Theor Appl Genet 100: 1018-1024.
Cantini, C., A. Cimato & G. Sani, 1999. Morphological evaluation of olive germplasm present in Tuscany region. Euphytica 109: 173-181.
Carriero, F., G. Fontanazza, F. Cellini & G. Giorio, 2002. Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.). Theor Appl Genet 104: 301-307.
Cipriani, G., M.T. Marrazzo, R. Marconi, A. Cimato & R. Testolin, 2002. Microsatellite markers isolated in olive are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars (Olea europaea L.). Theor Appl Genet 104: 223-228.
Cristoferi, G., A. Rotondi & M. Magli, 1997. Il germoplasma dell'olivo in Emilia-Romagna. Ed. ISTEA-CNR, Provincia di Rimini.
Del Rio, C. 1994. Preliminary agronomical characterization of 131 cultivars introduced in the olive germplasm bank of Cordoba in March 1987. Acta Hortic 356: 110-115.
Gemas, V.J., M.J. Rijo-Johansen, R. Tenreiro & P. Fevereiro, 2000. Inter-and intra-varietal analysis of three Olea europaea L. cultivars using the RAPD technique. J Hort Sci Biotechnol 75(3): 312-319.
Hansen, M., T. Kraft, M. Christiansson, N.O. Nilsson, 1999. Evaluation of AFLP in Beta. Theor Appl Genet 98: 845-852.
Hess, J., W. Kadereit & P. Vargas, 2000. The colonization history of Olea europaea L. in Macaronesia based on internal transcribed spacer 1 (ITS-1) sequences, randomly amplified polymorphic DNAs (RAPD), and intersimple sequence repeats (ISSR). Mol Ecol 9: 857-868.
Lumaret, R., M. Amane, N. Ouazzani & L. Baldoni, 2000. Chloroplast DNA variation in the cultivated and wild olive taxa of the genus Olea L. Theor Appl Genet 101: 547-553.
Mancuso, S. & F.P. Nicese, 1999. Identifying olive (Olea europaea) cultivars using Artificial Neural Networks. J Amer Soc Hort Sci 124(5): 527-531.
Mekuria, G.T., G.C. Collins & M. Sedgley, 1999. Genetic variability between different accessions of some common commercial olive cultivars. J Hort Sci Biotechnol 74(3): 309-314.
Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonuclease. Proc Natl Acad Sci USA 76: 5269-5273.
Rholf, F.J., 1995. NYSYS PC Numerical Taxonomy and Multivariate Analysis System. Vers. 1.80. Exter Software, Setauket, N.Y., USA.
Rotondi, A. & M. Magli, 1998. Valutazione comparativa della sensibilità a minime termiche critiche di cultivar di olivo della Romagna. Olivo & Olio 1: 48-54.
Rotondi, A. & M. Magli, 1999. Identificazione e classificazione di cultivar di olivo mediante analisi d'immagine. Olivo & Olio 10: 34-38.
Sefc, K.M., M.S. Lopes, D. Mendonca, M. Rodrigues Dos Santos, M. Laimer Da Camara Machado & A. Da Camara Machado, 2000. Identification of microsatellite loci in olive (Olea europaea) and their characterisation in Italian and Iberian olive trees. Mol Ecol 9: 1171-1193.
Trujillo, I., L Rallo & P. Arùs, 1995. Identifying olive cultivars by isozyme analysis. J Amer Soc Hort Sci 120 (2): 318-324.
Vlahov, G., A.D. Shaw & D.B. Kell, 1999. Use of 13 C Nuclear magnetic resonance distortionless enhancement by polarization transfer pulse sequence and multivariate analysis to discriminate olive oil cultivars. JAOCS Vol. 76(10): 1223-1231.
Wiesman, Z., N. Avidan, S. Lavee & B. Quebedeaux, 1998. Molecular characterization of common olive varieties in Israel and the West Bank using randomly amplified polymorphic DNA (RAPD) markers. J Am Soc Hort Sci 123: 837-841.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rotondi, A., Magli, M., Ricciolini, C. et al. Morphological and molecular analyses for the characterization of a group of Italian olive cultivars. Euphytica 132, 129–137 (2003). https://doi.org/10.1023/A:1024670321435
Issue Date:
DOI: https://doi.org/10.1023/A:1024670321435