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A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

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Abstract

We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

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References

  • Bailey MA, Parrott WA & Boerma HR (1993) Genotype effects on repetitive embryogenesis and plant regeneration of soybean.In Vitro Cell Dev. Biol. Plant 29: 102–108

    Google Scholar 

  • Barwale UB, Meyer MM & Widholm JM (1986) Screening of Glycine max (L.) Merr G. soma Sib. and Zucc. Genotypes for multiple shoot formation at the cotyledonary node. Theor. Appl. Genet. 72: 423–428

    Google Scholar 

  • Cai Q, Szarejko I & Maluszynski M (1992) The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breed. 109: 218–226

    Google Scholar 

  • Chong C & Taper CD (1972) Malus tissue cultures. I. Sorbitol (D-glucitol) as a carbon source for callus initiation and growth. Can. J. Bot. 50: 1399–1404

    Google Scholar 

  • Christou P, McCabe DE & Swain WF (1988) Stable transformation of soybean callus by DNA coated gold particles. Plant Physiol. 87: 671–674

    Google Scholar 

  • Coffin R, Taper CD & Chong C (1976) Sorbitol and sucrose are carbon sources for callus cultures of some species of Rosaceae. Can. J. Bot. 52: 2361–2364

    Google Scholar 

  • Donaldson PA & Simmonds DH (2000) Susceptibility of Agrobac-terium tumefaciens and cotyledonary node transformation in short-season soybean. Plant Cell Rep. 19: 478–484

    Google Scholar 

  • Delzer BW, Somers DA & Orf JH (1990) Agrobacterium susceptibility and plant regeneration of 10 soybean genotypes in maturity groups 00 to II. Crop Sci. 30: 320–322

    Google Scholar 

  • Finer JJ, Cheng T-S & Verma DPS (1996) Soybean transformation: technologies and progress. Soybean Genet. Mol. Biol. Biotechnol. 11: 249–262

    Google Scholar 

  • Garcia JL, Troncoso J, Sarmiento R & Troncoso A (2002) Influence of carbon source and concentration on the in vitro development of olive zygotic embryos and explants raised from them. Plant Cell Tiss. Org. Cult. 69: 95–100

    Google Scholar 

  • Graybosch RA, Edge ME & Deianny X (1987) Somaclonal variation in soybean plants regenerated from the cotyledonary node tissue culture system. Crop Sci. 27: 803–806

    Google Scholar 

  • Hinchee MAW, Conor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT & Horsch RB (1988) Production of transgenic soybean plants using Agrobac-terium mediated DNA transfer. Bio/Technology 6: 915–922

    Google Scholar 

  • Hu CY & Wang L (1999) In planta soybean transformation technologies developed in China: procedure, confirmation and field performance. In Vitro Cell Dev. Biol. Plant 35: 417–420

    Google Scholar 

  • Jahne A, Lazzeri PA, Jager-Gussen K & Lorz H (1991) Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor. Appl. Genet. 82: 74–80

    Google Scholar 

  • Klein TM & Jones TJ (1999) Methods of genetic transformation: the gene gun. In: Vasil IK (ed) Molecular Improvement of Cereal Crops (pp. 21–42). Kluwer Academic Publishers, The Netherlands

    Google Scholar 

  • Komatsuda T & Ko S-W (1990) Screening of soybean [Glycine max (L.) Merrill] genotypes for somatic embryo production from immature embryo. Jpn J. Breed. 40: 249–251

    Google Scholar 

  • Komatsuda T, Lee W & Oka S (1992) Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soybeans Glycine gracilis Skvortz and Glycine max (L.) Merr. Plant Cell Tiss. Org. Cult. 28: 103–113

    Google Scholar 

  • Komatsuda T & Ohyama K (1988) Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max. Plant Cell Rep. 75: 695–700

    Google Scholar 

  • Kuhlmann U & Foroughi-Wher B (1989) Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Rep. 8: 78–81

    Google Scholar 

  • Last DI & Brettel RIS (1990) Embryo yield in wheat anther culture is influenced by the choice of sugar in the culture medium. Plant Cell Rep. 9: 14–16

    Google Scholar 

  • Marchal J, Sens I & Teisson C (1992) Influence des sucre et de facteurs bioclimatiques sur la culture in vitro du bananier. Fruit 47: 17–24

    Google Scholar 

  • Meurer CA, Dinkins RD & Collins GB (1998) Factors affecting soybean cotyledonary node transformation. Plant Cell Rep. 18: 180–186

    Google Scholar 

  • Mhaske VB, Chengalrayan K & Hazra S (1998) Influence of osmotica and abscissic acid on triglyceride accumulation in peanut somatic embryos. Plant Cell Rep. 17: 742–746

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nadel BL, Altman A & Ziv M (1989) Regulation of somatic embryogenesis in celery cell suspensions. I. Promoting effects of mannitol on somatic embryo development. Plant Cell Tiss. Org. Cult. 18: 181–189

    Google Scholar 

  • Parrott WA, Hoffman LM, Hildebrand P, Williams EG & Collins GB (1989) Recovery of primary transformants of soybean. Plant Cell Rep. 7: 615–617

    Google Scholar 

  • Petersen KK, Hansen J & Krogstrup P (1999) Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus×ogiformis Honda ‘Giganteus'. Plant Cell Tiss. Org. Cult. 58: 189–197

    Google Scholar 

  • Pua E-C & Chong C (1984) Requirement for sorbitol (D-glucitol) as carbon source for in vitro propagation of Malus robusta. Can. J. Bot. 62: 1545–1549

    Google Scholar 

  • Sairam RV, Wilber C, Franklin J, Smith B, Bazil J, Hassel R, Frutiger K, Blakey CA, Vierling R & Goldman SL (2002) High frequency callus induction and plant regeneration in Tripsacum dactyloides (L.). In Vitro Cell. Dev. Biol. Plant 38: 435–440

    Google Scholar 

  • Shoemaker RC, Amberger LA & Palmer RG (1991) Effect of 2,4-dichlorophenoxyacetic acid concentrations on somatic embryogenesis and heritable variation in soybean [Glycine max (L.) Merr.]. In Vitro Cell Dev. Biol. Plant. 27: 84–88

    Google Scholar 

  • Vu JCV, Neidz RP & Yelonosky G (1993) Glycerol stimulation of chlorophyll synthesis, embryogenesis and carboxylation and sucrose metabolism enzymes in nucellar callus of ‘Hamlin’ sweet orange. Plant Cell Tiss. Org. Cult. 33: 75–80

    Google Scholar 

  • Walker DR & Parrot WA (2001) Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion. Plant Cell Tiss. Org. Cult. 64: 55–62

    Google Scholar 

  • Zhou H, Zheng Y & Konzak CF (1991) Osmotic potential of media affecting green plant percentage in wheat anther culture. Plant Cell Rep. 10: 63–66

    Google Scholar 

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Authors and Affiliations

  1. Plant Science Research Center, The University of Toledo, Toledo, Ohio, 43606, USA

    R.V. Sairam, G. Franklin, R. Hassel, B. Smith, K. Meeker, N. Kashikar, M. Parani, D. Al. Abed, S. Ismail, K. Berry & S.L. Goldman

Authors
  1. R.V. Sairam
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  2. G. Franklin
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  3. R. Hassel
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  4. B. Smith
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  5. K. Meeker
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  6. N. Kashikar
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  7. M. Parani
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  8. D. Al. Abed
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  9. S. Ismail
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  10. K. Berry
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  11. S.L. Goldman
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Correspondence to S.L. Goldman.

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Sairam, R., Franklin, G., Hassel, R. et al. A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell, Tissue and Organ Culture 75, 79–85 (2003). https://doi.org/10.1023/A:1024649122748

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