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Molecular cytogenetics in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding

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Abstract

Hop (Humulus lupulus L.) waskaryotyped by Fluorescence In SituHybridization (FISH) with probes 18S-25Sand 5S rDNA as well as DAPI banding andmeasurements of lengths of chromosome.Irrespective of the sex of the plants theseprobes revealed one signal near thetelomere of chromosome 6 and two signalseach on chromosomes 2 and 5.The shortest chromosome in a metaphaseplate of a male plant was designated to bechromosome Y. It displayed neither signalswith FISH nor DAPI bands. A middle sizedmetacentric chromosome with no telomericbut with an interstitial DAPI band on theshort arm was designated as X. Allautosomes displayed telomeric DAPI-positivebands. For the first time all mitotic hopchromosomes including the sex chromosomeswere identified by methods of molecularcytogenetics.

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Authors and Affiliations

  1. Department of Biotechnology, Timiryazev Agricultural Academy, Timiryazevskaya st. 44, 127550, Moscow, Russia

    G.I. Karlov & T.V. Danilova

  2. Institute of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Department of Plant Breeding and Biotechnology, D-70593, Stuttgart, Germany

    C. Horlemann & G. Weber

Authors
  1. G.I. Karlov
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  2. T.V. Danilova
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  3. C. Horlemann
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  4. G. Weber
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Karlov, G., Danilova, T., Horlemann, C. et al. Molecular cytogenetics in hop (Humulus lupulus L.) and identification of sex chromosomes by DAPI-banding. Euphytica 132, 185–190 (2003). https://doi.org/10.1023/A:1024646818324

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