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Overexpression of Plant Uncoupling Mitochondrial Protein in Transgenic Tobacco Increases Tolerance to Oxidative Stress

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Abstract

An Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northernblot, revealed variable levels of transgene expression. Antibody probing ofWestern blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significantincrease in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide thefirst biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.

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Authors and Affiliations

  1. Centro de Biologia Molecular e Engenharia Genética (CBMEG), Universidade Estadual de Campinas, C.P. 6010, 13083-970, Campinas, SP, Brazil

    Marcos Brandalise, Jiri Borecký & Paulo Arruda

  2. Departamento de Genética, IB, Universidade Estadual Paulista, Botucatu, SP, Brazil

    Ivan G. Maia

  3. Departamento de Patologia Clínica (NMCE), FCM, Universidade Estadual de Campinas, Campinas, SP, Brazil

    Aníbal E. Vercesi & Paulo Arruda

Authors
  1. Marcos Brandalise
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  2. Ivan G. Maia
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  3. Jiri Borecký
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  4. Aníbal E. Vercesi
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  5. Paulo Arruda
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Brandalise, M., Maia, I.G., Borecký, J. et al. Overexpression of Plant Uncoupling Mitochondrial Protein in Transgenic Tobacco Increases Tolerance to Oxidative Stress. J Bioenerg Biomembr 35, 203–209 (2003). https://doi.org/10.1023/A:1024603530043

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