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Cloning, expression and characterization of a β-agarase gene from a marine bacterium, Pseudomonas sp. SK38

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Abstract

A gene (pagA) encoding β-agarase from Pseudomonas sp. SK38 was cloned and expressed in Escherichia coli. The structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37 326 and has a signal peptide of 18 amino acids. The deduced amino acid sequence showed 57% and 58% homology to β-agarase from Pseudoalteromonas atalntica and Aeromonas sp., respectively. The recombinant enzyme was purified and biochemically characterized. The enzyme had maximum activity at pH 9 and 30 °C. It was stable at pHs from 8 to 9 and below 37 °C.

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Authors and Affiliations

  1. Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

    Nam-Young Kang, Yong-Lark Choi, Young-Su Cho & Young-Choon Lee

  2. Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyung-Pook, 780-350, Korea

    Nam-Young Kang & Cherol-Ho Kim

  3. Biotechnology Research Laboratory, Biohub Co. Ltd., Chinju, Gyeongnam, 660-701, Korea

    Beom-Kyu Kim, Beong-Sam Jeon & Jae-Young Cha

Authors
  1. Nam-Young Kang
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  2. Yong-Lark Choi
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  3. Young-Su Cho
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  4. Beom-Kyu Kim
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  5. Beong-Sam Jeon
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  7. Cherol-Ho Kim
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  8. Young-Choon Lee
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Correspondence to Young-Choon Lee.

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Kang, NY., Choi, YL., Cho, YS. et al. Cloning, expression and characterization of a β-agarase gene from a marine bacterium, Pseudomonas sp. SK38. Biotechnology Letters 25, 1165–1170 (2003). https://doi.org/10.1023/A:1024586207392

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