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Isolation of myo-Inositol from Solutions by Enzymatic Biotransformation

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Abstract

The transferase reaction between phospholipids and inositol catalyzed by phospholipase D was studied at interfaces in water–organic solvent systems. Optimum conditions were determined for phosphatidylinositol synthesis in heterogeneous water–organic solvent systems. Hydrophobic components (phospholipids) were readily separated from water-soluble products (alcohols) in systems with organic solvents. In the hexane–water system, addition of methanol (an alcohol substrate) to the reaction medium displaced myo-inositol from the molecule of phosphatidylinositol. myo-Inositol was isolated from the mixture of its isomers using a two-step transferase reaction catalyzed by phospholipase D.

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Authors and Affiliations

  1. Mirzo Ulugbek National University of Uzbekistan, Tashkent, 700174, Uzbekistan

    E. N. Reut & M. M. Rakhimov

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  1. E. N. Reut
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  2. M. M. Rakhimov
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Reut, E.N., Rakhimov, M.M. Isolation of myo-Inositol from Solutions by Enzymatic Biotransformation. Applied Biochemistry and Microbiology 39, 363–367 (2003). https://doi.org/10.1023/A:1024560232500

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