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Determination of the Primary and Fine Structures of a Galactomannan from the Seed of Gleditsia triacanthos f. inermis L.

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Abstract

Galactomannan, a polysaccharide with a molecular weight of 660 kDa, was isolated for the first time from the seed of Gleditsia triacanthos f. inermis (yield, 15.4%). Its aqueous solutions were optically active ([α] D = +31.0°) and highly viscous ([η] = 578 ml/g). Analysis of this heteropolysaccharide using chemical, enzymatic, and chromatographic procedures, as well as IR and 13C NMR spectroscopy, showed that it consists of D-mannopyranose and D-galactopyranose residues (molar ratio, 2.42 : 1). The main chain of this galactomannan comprises 1,4-β-D-mannopyranose residues, 41% of which are substituted at C6 with single residues of α-D-galactopyranose. The probability of occurrence in the chain of mannobiose units substituted otherwise, determined experimentally, was 0.16 for the Man–Man unit, 0.50 for the Gal(Man–Man) and (Man–Man)Gal units, and 0.34 for the disubstituted Gal(Man–Man)Gal unit.

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Authors and Affiliations

  1. Russian Academy of Sciences, Bach Institute of Biochemistry, Leninskii pr. 33, Moscow, 119071, Russia

    A. V. Egorov, N. M. Mestechkina & V. D. Shcherbukhin

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  1. A. V. Egorov
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  2. N. M. Mestechkina
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  3. V. D. Shcherbukhin
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Egorov, A.V., Mestechkina, N.M. & Shcherbukhin, V.D. Determination of the Primary and Fine Structures of a Galactomannan from the Seed of Gleditsia triacanthos f. inermis L.. Applied Biochemistry and Microbiology 39, 398–402 (2003). https://doi.org/10.1023/A:1024524719296

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